Assays & Kits

Fructose-1,6-diphosphate (FDP) is an important intermediate product in the glycolysis process. It can regulate a variety of enzymes, improve cell energy metabolism, increase energy utilization, anti-arrhythmia and anti-tissue peroxidation. FDP is widely used in clinical medicine. CheKine™ Micro Fructose-1,6 Diphosphate (FDP) Content Assay Kit can be used to detect biological samples such as animal tissue, bacteria or cells, serum or plasma. In the kit, aldolase catalyzes the cleavage of fructose 1,6-diphosphate. The product reacts with 2,4- dinitrophenylhydrazine in acid medium to form 2, 4-dinitrophenylhydrazone, which is dark red in alkaline solution and has a characteristic absorption peak at 540 nm.

The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix. CheKine™ Micro Plant Dehydrogenase (PDHA) Activity Assay Kit can be used to detect biological samples such as plant tissues. In this kit, The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenyl formazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorptionpeak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.

Asparagine synthetase (AS) is a widely distributed enzyme in living organisms belonging to the class of amino transferases. It catalyzes the transfer of an amine group from glutamine to aspartic acid. When a plant is subjected to ammonia toxicity, the formation of asparagine serves as a detoxification mechanism. CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit is designed to quantify asparagine synthetase activity in animal and plant tissues, bacterial and cellular samples, as well as in serum (plasma). The assay principle relies on the AS enzyme's ability to catalyze the hydrolysis of L-asparagine into L-aspartic acid and ammonia. By employing Nessler's reagent to detect the rate of ammonia accumulation, the enzymatic activity of AS can be determined.

cfDNA Purification Kit (Pre-filled) is designed for the optimal automated extraction of DNA from human serum and plasma.

Fungi DNA Purification Kit (Pre-filled) is designed for the optimal automated extraction of DNA from wide ranges of fungi samples such as the yeast and filamentous fungi.

Forensic DNA Purification Kit (Pre-filled) is designed for the optimal automated extraction of DNA from a broad range of forensic samples, including human blood stain, dried blood spot, hair follicle, semen, cigarette, chewing gum, and nail.

Stool DNA Purification Kit (Pre-filled) is designed for the optimal automated extraction of DNA from stool of human and other species.