Assays & Kits

Screen for 3CL protease inhibitors in a BSL-1 environment. This is an extremely bright and robust 3CLpro assay, packaged in BacMam for mammalian cell expression. Green fluorescence increases when 3CLpro is successfully inhibited. Kit comes with the 3CLglowUp biosensor BacMam, 3CLPro enzyme BacMam, and 500mM Sodium butyrate in H2O.

The 3CLglowLive Assay kit is well suited to experiments using the live SARS-CoV-2 virus. It is dim at baseline but fluoresces green in the presence of 3CL protease activity, an indicator of SARS-CoV-2 viral replication. The kit includes 2 BacMam viruses - one expressing the 3CLglowLive biosensor and the other a 3CLpro enzyme, which could be used to test the biosensor and optimize fluorescence detection settings.

Fluorescent biosensor for cyclic AMP (cAMP) packaged in Montana Molecular's improved Big Sky BacMam vector. Measure signaling kinetics, screen GPCRs for Gs activity, and more with the live cell assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. BacMam provides robust expression in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 15mL and 30mL sizes are also available.

Fluorescent biosensor for cyclic AMP (cAMP) packaged in Montana Molecular's improved Big Sky BacMam vector. Measure signaling kinetics, screen GPCRs for Gs activity, and more with the live cell assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. BacMam provides robust expression in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 5mL and 30mL sizes are also available.

Fluorescent biosensor for cyclic AMP (cAMP) packaged in Montana Molecular's improved Big Sky BacMam vector. Measure signaling kinetics, screen GPCRs for Gs activity, and more with the live cell assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. BacMam provides robust expression in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 5mL and 15mL sizes are also available.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in Montana Molecular's improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

Fluorescent Arrestin Recruitment Assay for the Melanocortin 4 Receptor (MC4R). Packaged in BacMam to be expressed in living cells. Gather real time signaling kinetics, detect agonist bias, and more with plate readers or fluorescence microscopes.

Fluorescent Arrestin Recruitment assay for the C-C Chemokine Receptor Type 5 (CCR5). Deliver the biosensor and unmodified receptor to living cells, and detect signaling kinetics on automated plate readers of fluorescence microscopes. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, CCR5 BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Several other receptor-specific kits are available, and other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.