Assays & Kits

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. This is an update to U0200G, packaged in Montana Molecular's new Big Sky BacMam vector. This change provides 3-4x improvements in transduction efficiency in cell types such as HEK293 and CHO. BacMam also robustly transduces many iPSCs and primary cultures. Increasing green fluorescence indicates cAMP increase. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 15mL and 30mL sizes are also available.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. This is an update to U0200G, packaged in Montana Molecular's new Big Sky BacMam vector. This change provides 3-4x improvements in transduction efficiency in cell types such as HEK293 and CHO. BacMam also robustly transduces many iPSCs and primary cultures. Increasing green fluorescence indicates cAMP increase. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 5mL and 30mL sizes are also available.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. This is an update to U0200G, packaged in Montana Molecular's new Big Sky BacMam vector. This change provides 3-4x improvements in transduction efficiency in cell types such as HEK293 and CHO. BacMam also robustly transduces many iPSCs and primary cultures. Increasing green fluorescence indicates cAMP increase. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 5mL and 15mL sizes are also available.

Fluorescent biosensor for cyclic AMP (cAMP) packaged in Montana Molecular's improved Big Sky BacMam vector. This is an update to #U0200R, yielding 3-4x increases in expression in HEK293 and CHO cells. Measure signaling kinetics, screen GPCRs for Gs activity, and more with the live cell assay. Increasing red fluorescence indicates cAMP increase. BacMam provides robust expression in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 15mL size is also available.

Fluorescent biosensor for cyclic AMP (cAMP) packaged in Montana Molecular's improved Big Sky BacMam vector. This is an update to #U0200R, yielding 3-4x increases in expression in HEK293 and CHO cells. Measure signaling kinetics, screen GPCRs for Gs activity, and more with the live cell assay. Increasing red fluorescence indicates cAMP increase. BacMam provides robust expression in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 5mL size is also available.

U0261G is a fusion of the complete AKAP18a protein and the cADDis cAMP biosensor. In HEK 293 cells, the AKAP18a-targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0262G is a fusion of the complete AKAP18b protein to the cADDis biosensor for cAMP. In HEK293T cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0263G is a fusion of the complete AKAP1 protein to the cADDis cAMP biosensor. In HEK293 cells, the AKAP1-targeted cAMP sensor localizes to the mitochondria. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0264G is a fusion of the complete AKAP79 protein to the cADDis biosensor. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).