Assays & Kits

U0266G is a fusion of the complete AKAP12 protein and the cADDis biosensor. In HEK293 cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

U0267G is a fusion of the complete smAKAP protein and the cADDis biosensor. In HEK293 cells the fluorescence is targeted to the plasma membrane. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in Montana Molecular's improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in an improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the red fluorescent R-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in an improved Big Sky BacMam vector for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the R-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O).

A biosensor for the IRE1-XBP1 arm of the UPR. Measure both the onset and reversal of ER stress from chemical stressor, genetic stressors, and more. This is an update to #U0900G and #U0901G. The bright mNeongreen fluorescent protein is produced when cells are undergoing ER stress, while the constitutively expressed Montana Painbrush RFP provides a marker for sensor expression and cell health. Measure green-only or green/red ratios with high signal-to-noise on plate readers or fluorescence imaging systems. Monitor living cells over time with no need to lyse or fix. Packaged in Montana Molecular's improved Big Sky BacMam vector. 15mL and 30mL sizes are also available.

A biosensor for the IRE1-XBP1 arm of the UPR. Measure both the onset and reversal of ER stress from chemical stressor, genetic stressors, and more. This is an update to #U0900G and #U0901G. The bright mNeongreen fluorescent protein is produced when cells are undergoing ER stress, while the constitutively expressed Montana Painbrush RFP provides a marker for sensor expression and cell health. Measure green-only or green/red ratios with high signal-to-noise on plate readers or fluorescence imaging systems. Monitor living cells over time with no need to lyse or fix. Packaged in Montana Molecular's improved Big Sky BacMam vector. 5mL and 30mL sizes are also available.

A biosensor for the IRE1-XBP1 arm of the UPR. Measure both the onset and reversal of ER stress from chemical stressor, genetic stressors, and more. This is an update to products #U0900G and #U0901G. The bright mNeongreen fluorescent protein is produced when cells are undergoing ER stress, while the constitutively expressed Montana Painbrush RFP provides a marker for sensor expression and cell health. Measure green-only or green/red ratios with high signal-to-noise on plate readers or fluorescence imaging systems. Monitor living cells over time with no need to lyse or fix. Packaged in Montana Molecular's improved Big Sky BacMam vector. 15mL and 30mL sizes are also available.