Assays & Kits

Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE7A, also known as high affinity cAMP-specific phosphodiesterase 7A, has been implicated in cardiovascular function and fertility. The Rat PDE7A Assay Kit is designed for identification of inhibitors of Rat PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE7A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit

The Mouse PDE10A Assay Kit is designed for identification of inhibitors of Mouse PDE10A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE10A to the binding agent. The key to the Mouse PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

The Mouse PDE7A Assay Kit is designed for identification of inhibitors of Mouse PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE7A to the binding agent. The key to the Mouse PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

The Mouse PDE2A Assay Kit is designed for identification of inhibitors of Mouse PDE2A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE2A to the binding agent. The key to the Mouse PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE2A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE2A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

TMB (3,3', 5, 5' - tetramethylbenzidine) Substrate develops a deep blue color in the presence of horseradish peroxidase (HRP)- labeled conjugates measurable at 650 nm and turns yellow when stopped by acidification and read at 450 nm. Optimized for Use With: SARS-CoV-1 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience, #78012)</br> Spike S1 (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience, #79954)</br> SARS-CoV-2 IgG Detection Kit (Colorimetric Trimer Anti-Spike IgG detection) (BPS Bioscience, #79975)</br> SARS-CoV-2 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience, #79999)

The Mouse PDE7B Assay Kit is designed for identification of inhibitors of Mouse PDE7B using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE7B to the binding agent. The key to the Mouse PDE7B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE7B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE7B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

The CTLA4:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-2-biotin interaction. The key to this kit is the high sensitivity of detection of CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-2-biotin is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

ALKBH5 (AlkB Homolog 5, RNA Demethylase) is an enzyme that removes N6-Methyladenosine (m^6A) from target mRNAs. ALKBH5 has been connected to various cancers, viral replication, and metabolic diseases while also being shown to remove the alkylation damage and restore adenine in DNA.

c-Mer (MERTK) is a member of the TAM receptor kinases and plays an important role in cell proliferation/survival, adhesion/migration, and regulation of inflammatory cytokine release. Along with other members of this family, Axl and TYRO3, c-Mer is part of an emerging class of innate immune checkpoints participating in antitumoral immunity. Thus, it has been suggested that c-Mer inhibition could be a promising immunotherapeutic approach in cancer treatment. The c-Mer Kinase Assay Kit is designed to measure c-Mer activity for screening and profiling applications using ADPGlo® as a detection reagent.