Assays & Kits

The BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BAFF:BCMA signaling.The key to this kit is the high sensitivity of detection of biotin-labeled BCMA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BAFF is coated on a 96-well plate. Next, BCMA is incubated with BAFF on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

SYK of the nonreceptor tyrosine kinases plays a role in autoimmune diseases like Epstein Barr virus and hematopoietic malignancies. The SYK Assay Kit is designed to measure SYK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

The CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of CD38 for screening and profiling applications.

The ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of ICOS:ICOSL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled ICOS by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ICOSL is coated on a 96-well plate. Next, ICOS is incubated with ICOSL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Cyclin-dependent kinase 4 (CDK4) assembles with the "D" type of Cyclins, e.g. cyclins D1, D2, and D3, and play a pivotal role in the cell cycle entering the S phase. Dysregulation of the activity of CDK4 is related to many different cancers, indicating CDK4 inhibitors as promising anticancer treatments. In fact, multiple CDK4 inhibitors, including Palbociclib, were approved by the FDA recently. The CDK4 Assay Kit is designed to measure CDK4/CyclinD3 activity for screening and profiling applications, using Kinase-Glo® Max as a detection reagent.

SRC is a member of the nonreceptor tyrosine kinases that plays a role in many cellular functions, including cell adhesion, growth, and differentiation. SRC has been implicated in diseases such as chronic kidney disease and metastatic bone disease. The SRC Assay Kit is designed to measure SRC activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The SRC Assay Kit comes in a convenient 96-well format, with enough purified recombinant SRC enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

Proto-oncogene tyrosine-protein kinase YES1 has been implicated in regulation of cell growth and survival, apoptosis, cell-cell adhesion, and differentiation. It has been identified as a potential target in basal-like breast cancers. The YES1 Assay Kit is designed to measure YES1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The YES1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant YES1 enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

The Mouse CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of mouse CD38 for screening and profiling applications. The CD38 assay kit comes in a convenient 96-well format, with purified recombinant murine CD38 enzyme, its substrate N6-etheno-NAD (ε-NAD), and CD38 assay buffer for 96 enzyme reactions. In addition, the kit includes the CD38 inhibitor apigenin for use as a control inhibitor.

The LIGHT:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LIGHT is coated on a 96-well plate. Next, HVEM is incubated with LIGHT on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.