Results for Assays & Kits ( 14552 )
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Protein-protein crosslinking can be achieved by the hydrazone method and thiol-maleimide method. <br /> Thiol-maleimide method is commonly performed with a bifunctional linker, such as the commonly used SMCC. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause a significant amount of homo-crosslinking. The maleimide conjugates can re-arrange, hydrolyze, or exchange in the presence of thiols/cysteine. <br /> <br /> In the hydrazone coupling method, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> The novel technology of BroadPharm's MagicLink™ has been engineered to solves all the problems above and allow rapid and quantifiable conjugation of all classes
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Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.<br /> The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (?12 carbons) onto the core fluorophore.