Assays & Kits

MagicLink&trade; Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. </p> This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get

MagicLink&trade; Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. </p> This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get

BroadPharm's SN-38 Antibody Conjugation kit attaches the drug, SN-38 to antibody. The kit is designed to utilize the linker's maleimide group to react and attach to thiol groups on the antibody.</p> The linker- SN-38 is made by SN38 conjugated to the lysosomally cleavable dipeptide, valine-citrulline (vc) linker. SN-38 is the active metabolite of the topoisomerase-I (topo-I) inhibitor Irinotecan (CPT-11 ), and it stabilizes the complex between topo-I and DNA which collide with moving DNA replication forks, eventually leading to double stranded DNA damage.</p> <ol type='A'> Kit Features: <li>Protocol: easy to follow, to label IgG with SN38 with minimum exposure to the chemotherapeutic drug</li> <li>Linkage: releasable linkage</li> <li>Fast process: <1 h hands-on time, 6 h for whole process. </li> <li>Convient: All reagents and supplies included for preparation purification, and storage</li> <li>DAR: average 3-8</li> <li>Efficient: More than 99% conjugated products by SEC and is f

BroadPharm's SN-38 Antibody Conjugation kit attaches the drug, SN-38 to antibody. The kit is designed to utilize the linker's maleimide group to react and attach to thiol groups on the antibody.</p> The linker- SN-38 is made by SN38 conjugated to the lysosomally cleavable dipeptide, valine-citrulline (vc) linker. SN-38 is the active metabolite of the topoisomerase-I (topo-I) inhibitor Irinotecan (CPT-11 ), and it stabilizes the complex between topo-I and DNA which collide with moving DNA replication forks, eventually leading to double stranded DNA damage.</p> <ol type='A'> Kit Features: <li>Protocol: easy to follow, to label IgG with SN38 with minimum exposure to the chemotherapeutic drug</li> <li>Linkage: releasable linkage</li> <li>Fast process: <1 h hands-on time, 6 h for whole process. </li> <li>Convient: All reagents and supplies included for preparation purification, and storage</li> <li>DAR: average 3-8</li> <li>Efficient: More than 99% conjugated products by SEC and is f

The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit does not include E. coli cells; however, another version of the kit includes E.coli chemically competent cells for transformation (BPS Bioscience #78532).

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit does not include E. coli cells; however, another version of the kit includes E.coli chemically competent cells for transformation (BPS Bioscience #78532).

The Quick PCR™ Plus Assembly Kit is used as a molecular cloning tool to assemble long DNA fragment from multiple smaller fragments, or to insert DNA into a plasmid in a single reaction. The main kit component is a ready-to-use mix of enzymes in reaction buffer at a 2-fold concentration. This kit also includes chemically competent E. coli cells (another version of the kit does not include competent cells, BPS Bioscience #78531).