Assays & Kits

The breast tumor kinase (BRK) Kinase Assay Kit is designed to measure BRK tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. 

The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions. The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.

The rat histone deacetylase 6 (HDAC6) Assay Kit is a 96-well fluorogenic assay designed to measure rat HDAC6 enzymatic activity for screening and profiling applications. In addition, the kit includes a potent HDAC inhibitor, Trichostatin A (TSA), for use as control. The Fluorogenic Rat HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. A fluorescent dye is conjugated to a peptide containing acetyl-lysine. The fluorescence of the conjugated dye is quenched by the peptide. After treatment of the peptide with an HDAC, a developer solution specific for non-acetylated lysine is added to the reaction. If the acetyl group has been removed from the lysine, the developer releases the dye, allowing for fluorescence. In this assay, fluorescence directly correlates with HDAC activity.

The NIMA-related Kinase 7 (NEK7) Kinase Assay Kit is designed to measure NEK7 serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. 

The TNF receptor associated factor 6 (TRAF6) TR-FRET Assay Kit is a sensitive TR-FRET Assay Kit, designed to measure TRAF6 (amino acids 51-159) auto-ubiquitination activity in a homogeneous 384 reaction format. This assay measures mono- or poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps and is especially suitable for high throughput screening (HTS) applications as well as real-time analyses. Read out requires a fluorescent plate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET). The assay takes advantage of biotinylated ubiquitin, which is linked to TRAF6 during auto-ubiquitination. The streptavidin-conjugated acceptor binds to ubiquitin-biotin while a Terbium-conjugated anti-GST antibody donor binds to GST-tagged TRAF6, allowing TR-FRET pairing. The resulting TR-FRET signal is directly proportional to TRAF6 mono or poly-ubiquitination.

The Dicer Fluorogenic Assay Kit is a homogeneous 384-well assay designed to measure the double-stranded-RNA processing activity of Dicer for screening and profiling applications. The Dicer Fluorogenic Assay Kit contains enough purified Dicer, substrate, and assay buffer for 384 reactions. The assay relies on an RNA duplex imitating pre-miRNA, containing a conjugated fluorophore and quencher pair. Fluorescence of the dye is effectively quenched by the proximity of the quencher in the intact RNA duplex. The endoribonuclease Dicer processes the pre-miRNA into a mature 22 bp double-stranded miRNA by cleaving the 5' end which is conjugated to the dye, releasing it from the miRNA. Separated from the quencher, the dye emits fluorescence with excitation/emission maxima of λ=647/ 665 nm. Dicer activity is proportional to the increase in fluorescence.

The Lumi-Verse™ VEGFR2 (KDR) Kinase Assay Kit is designed to measure Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) tyrosine kinase activity for screening and profiling applications, using the highly sensitive ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant VEGFR2 (KDR) kinase domain (amino acids 805-1356), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Ubiquitin specific peptidase 2 (USP2) Inhibitor Screening Assay Kit is a homogeneous fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP2 for screening and profiling applications. The kit contains enough purified USP2 recombinant protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. To determine the effect of an inhibitor on USP2 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). In the conjugated form, the energy emitted from fluorochrome AMC is q

The MMP7 (Matrix MetalloProteinase 7) Fluorogenic Assay Kit is designed to measure MMP7 protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.