Assays & Kits

The E. coli in vivo Biotinylation Kit (without BirA Competent Cells) comes in a convenient, easy-to-use format and contains the components necessary to label biotin-acceptor peptides with biotin expressed in E. coli. strain BL21, a chemically competent E. coli B strain. This kit contains sufficient reagents to label 10 x 1 L of E. coli culture. BirA-Transformed chemically competent E. coli cells (BPS Bioscience #27462) are not provided with this kit and must be purchased separately. 

The PARP3 Chemiluminescent Assay Kit is designed to measure PARP3 activity for screening and profiling applications. The PARP3 assay kit 96-well format contains enough purified PARP3 enzyme, histone mixture, activated DNA template, PARP assay buffer and necessary reagents for 100 or 400 enzyme reactions. The key to the PARP3 Chemiluminescent Assay Kit is the biotinylated NAD⁺ and involves 3 reactions. First, histone proteins are coated on a plate. Next, a biotinylated NAD⁺ mix (termed PARP Substrate Mixture) is incubated with the enzyme and an activated DNA template. Finally, streptavidin-HRP is added, followed by ELISA ECL substrate to produce a chemiluminescence signal that can be measured using a chemiluminescence plate reader. *NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

The PARP3 Chemiluminescent Assay Kit is designed to measure PARP3 activity for screening and profiling applications. The PARP3 assay kit 96-well format contains enough purified PARP3 enzyme, histone mixture, activated DNA template, PARP assay buffer and necessary reagents for 100 or 400 enzyme reactions. The key to the PARP3 Chemiluminescent Assay Kit is the biotinylated NAD⁺ and involves 3 reactions. First, histone proteins are coated on a plate. Next, a biotinylated NAD⁺ mix (termed PARP Substrate Mixture) is incubated with the enzyme and an activated DNA template. Finally, streptavidin-HRP is added, followed by ELISA ECL substrate to produce a chemiluminescence signal that can be measured using a chemiluminescence plate reader. *NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

The RayBiotech Protein Oxidation (Carbonyl) Assay Kit measures protein carbonyl content in plasma, serum, cell lysates and other biological liquid samples using a 96-well microplate.

Sandwich TR-FRET immunoassay for quantitative detection of Human Pro-Collagen I alpha 1 (COL1A1) in cell culture supernatants. The kit does not include a recombinant Human COL1A1 standard, which must be purchased separately from R&D Systems (catalogue number 6220-CL).

Sandwich TR-FRET immunoassay for quantitative detection of Human Pro-Collagen I alpha 1 (COL1A1) in cell culture supernatants. The kit does not include a recombinant Human COL1A1 standard, which must be purchased separately from R&D Systems (catalogue number 6220-CL).

Sandwich TR-FRET immunoassay for quantitative detection of Human Pro-Collagen I alpha 1 (COL1A1) in cell culture supernatants. The kit does not include a recombinant Human COL1A1 standard, which must be purchased separately from R&D Systems (catalogue number 6220-CL).

Sandwich TR-FRET immunoassay for quantitative detection of Human Pro-Collagen I alpha 1 (COL1A1) in cell culture supernatants. The kit does not include a recombinant Human COL1A1 standard, which must be purchased separately from R&D Systems (catalogue number 6220-CL).

Sandwich TR-FRET immunoassay for quantitative detection of Human Pro-Collagen I alpha 1 (COL1A1) in cell culture supernatants. The kit does not include a recombinant Human COL1A1 standard, which must be purchased separately from R&D Systems (catalogue number 6220-CL).