Assays & Kits

Human PXR activation and cell viability in 96 well format. This product is available for laboratory services; please contact your local office to discuss and build your project.

Human CAR1 activation and cell viability in 96 well format. This product is available for laboratory services; please contact your local office to discuss and build your project.

Rat PXR activation and cell viability in 96 well format. This product is available for laboratory services; please contact your local office to discuss and build your project.

A kinetic biosensor assay for arrestin recruitment converts conformational changes in arrestin-3 (β-arrestin-2) to changes in fluorescence intensity. This kit detects arrestin recruitment at the unmodified µ-Opioid Receptor (MOR). The Borealis Arrestin Sensor, and MOR, are packaged in a BacMam viral vector for easy delivery to most cell types. Validated for use on fluorescence imaging systems and automated fluorescence plate readers.

Sircol 2.0 is the newest member of Biocolor's family of collagen kits, designed to simplify quantative measurement of soluble collagens from in-vivo & in-vitro samples. It provides enhanced sensitivity and specificity for accurate collagen measurement, surpassing the performance of our previous soluble collagen kit.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.

The PARG Fluorogenic Assay Kit is a high-throughput, homogeneous 96-well or 384-well assay designed to measure the hydrolase activity of Poly (ADP-ribose) glycohydrolase (PARG) for screening and profiling applications, using a simple and straightforward fluorogenic assay. The PARG Fluorogenic Assay Kit contains enough purified recombinant PARG enzyme, substrate, and assay buffer for 100 or 400 enzyme reactions. <p style="text-align: center;"><img src="{{media url="wysiwyg/PARPs/78858.png"}}" alt="" width="388" height="188" /> Figure 1: Illustration of the assay principle. PARG is incubated with a fluorogenic ADP-ribose substrate in which the fluorophore is quenched by the presence of the ribose. PARG-mediated hydrolysis of the substrate between the ribose and the fluorochrome releases fluorescence that can be detected at λ=502 nm (excitation at λ=385 nm). Fluorescence intensity is directly proportional to PARG hydrolase activity. 

The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.