Assays & Kits

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized. The standard FISH technique, however, often suffers from limitations, namely poor signal to background n

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized. The standard FISH technique, however, often suffers from limitations, namely poor signal to background n

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized. The standard FISH technique, however, often suffers from limitations, namely poor signal to background n

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized. The standard FISH technique, however, often suffers from limitations, namely poor signal to background n

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments. The kits are designed for flow cytometry analysis. They contain special reagents and a dedicated protocol for read-outs using flow cytometry on cells in suspension. The kits are available for the followin