Assays & Kits

The Diablo:XIAP Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Diablo (also named Smac) and E3 ubiquitin-protein ligase XIAP (X-linked inhibitor of apoptosis, also known as mammalian IAP homolog A) for screening and profiling applications. The Diablo:XIAP Inhibitor Screening Assay Kit comes with enough purified Diablo (amino acids 56-239) and XIAP proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with purified Diablo protein. After coating and blocking, XIAP is added in an optimized assay buffer. Next, unbound XIAP is washed away and the plate is incubated with a primary antibody, followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the binding of XIAP to Diablo.

The DCAF1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure DCAF1 (DDB1 and CUL4-associated Factor 1) complex auto-ubiquitination activity. It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF1. The kit contains enough purified DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions.

The Chemi-Verse™ CDC7 Kinase Assay Kit is designed to measure CDC7 (cell division cycle 7-related protein kinase) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified CDC7, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The DCAF1 Binding Assay Kit is a fluorescence polarization (FP), homogeneous, 96-well assay kit designed for the screening and profiling of molecules that inhibit the binding of the DCAF1 (DDB1 and CUL4 Associated Factor 1) Complex to a high affinity DCAF1 fluorescent probe. The kit contains enough purified recombinant DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, fluorescent probe and assay buffer for 100 reactions. The assay uses a DCAF1 fluorescent probe. This is a very small molecule that rotates fast (low FP). This probe is incubated with the DCAF1 complex leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to binding of DCAF1 to the fluorescent probe. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: <a href="https://bpsbioscience.com/fluorescence-polarization-assays-princi

The Chemi-Verse™ RSK4 Kinase Assay Kit is designed to measure RSK4 (ribosomal S6 kinase 4) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified RSK4, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ PIM1 Kinase Assay Kit is designed to measure PIM1 (Proviral Integration of Molony murine leukemia virus 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified PIM1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The CD155:TIGIT [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed for screening and profiling molecules that block the binding between CD155 (cluster of differentiation 55) and TIGIT (T-cell immunoreceptor with Ig and ITIM domains). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TIGIT (amino acids 22-141), CD155 (amino acids 27-343), streptavidin-labeled HRP, and assay buffer for 100 reactions. A 96-well plate is coated with the human recombinant CD155 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-TIGIT, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal is measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD155 to TIGIT.

The Activin A: Activin RIIB [Biotinylated] Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Activin A and Activin RIIB (receptor IIB), also known as ACVR2B, protein for screening and profiling applications. This kit comes with enough purified Activin A (amino acids 311-426) and biotinylated Activin Receptor IIB (amino acids 19-137), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with Activin A protein. After coating and blocking, biotinylated Activin RIIB, also known as ACVR2B, is added in an optimized assay buffer. Next, unbound biotinylated Activin RIIB is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of Activin RIIB binding to Activin A.

The CD155:CD96 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed for screening and profiling molecules that block the binding between CD155 (cluster of differentiation 155) and CD96 (cluster of differentiation 96). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD96 (amino acids 22-503), CD155 (amino acids 27-343), streptavidin-labeled HRP, and assay buffer for 100 reactions. A 96-well plate is coated with CD155 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD96, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD155 to CD96.