Assays & Kits

The Activin A: Activin RIIB [Biotinylated] Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Activin A and Activin RIIB (receptor IIB), also known as ACVR2B, protein for screening and profiling applications. This kit comes with enough purified Activin A (amino acids 311-426) and biotinylated Activin Receptor IIB (amino acids 19-137), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with Activin A protein. After coating and blocking, biotinylated Activin RIIB, also known as ACVR2B, is added in an optimized assay buffer. Next, unbound biotinylated Activin RIIB is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of Activin RIIB binding to Activin A.

The CD155:CD96 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed for screening and profiling molecules that block the binding between CD155 (cluster of differentiation 155) and CD96 (cluster of differentiation 96). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD96 (amino acids 22-503), CD155 (amino acids 27-343), streptavidin-labeled HRP, and assay buffer for 100 reactions. A 96-well plate is coated with CD155 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD96, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD155 to CD96.

The BAFF:BAFFR [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between BAFF (B-cell Activating Factor) and BAFFR (BAFF receptor). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled BAFFR (amino acids 23-71), BAFF (amino acids 134-285), streptavidin-HRP, and assay buffer for 100 reactions. A 96-well plate is coated with BAFF protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-BAFFR, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of BAFF to BAFFR.

The ADAM9 Fluorogenic Assay Kit is designed to measure ADAM9 (A disintegrin and a metalloprotease 9) protease activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant ADAM9, fluorogenic substrate and solution, and assay buffer for 100 enzyme reactions.  

The Chemi-Verse™ JAK1 Kinase Assay Kit is designed to measure JAK1 (Janus kinase 1) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified JAK1 (amino acids 866-1154), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ JAK2 Kinase Assay Kit is designed to measure JAK2 (Janus Kinase 2) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified JAK2 (JH1 domain) (amino acids 808-1132), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ JAK3 Kinase Assay Kit is designed to measure JAK3 (Janus Kinase 3) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified JAK3 (amino acids 811-1124), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ TBK1 Kinase Assay Kit is designed to measure TBK1 (TANK-binding kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified TBK1 (amino acids 2-279), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ HER2 Kinase Assay Kit is designed to measure HER2 (human epidermal growth factor receptor 2) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified HER2 (amino acids 679-1255), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.