Assays & Kits

The TSLPR:TSLP [Biotinylated] Small Molecule Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling small molecules that block the binding between TSLPR (thymic stromal lymphopoietin receptor) and TSLP. This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TSLP (amino acids 29-159), human TSLPR (amino acids 25-231), streptavidin-HRP, and assay buffer for 100 reactions. A 96-well plate is coated with TSLPR protein. After coating and blocking, TSLP is added in an optimized assay buffer. Unbound TSLP is washed away, and the plate is incubated with streptavidin-HRP. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of TSLP binding to TSLPR.

Easy identification of cell death pathways in combination with other Dojindo’s kits, Multiple indicators can be measured in combination on the same plate, and Accurate plate assay without the need for washing.

The RayBio® Human TBMNK Flow Cytometry Kit is designed to determine the percentages of mature human lymphocyte and monocyte subsets in peripheral whole blood (WB).

The RayBio® Human TBMNK Flow Cytometry Kit is designed to determine the percentages of mature human lymphocyte and monocyte subsets in peripheral whole blood (WB).

Measure Gi signaling kinetics or activation with this live cell cAMP assay. This is a update to #X0200G, packaged in Big Sky BacMam providing a 3-4x increase in expression in cell types including HEK293 and CHO cells. BacMam also robustly transduces many iPSC and primary cultures, including neuronal and cardiac cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (D2R BacMam and 2mM quinpirole in H2O). Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay. 15mL and 30mL sizes are also available.

Measure Gi signaling kinetics or activation with this live cell cAMP assay. This is a update to #X0200G, packaged in Big Sky BacMam providing a 3-4x increase in expression in cell types including HEK293 and CHO cells. BacMam also robustly transduces many iPSC and primary cultures, including neuronal and cardiac cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (D2R BacMam and 2mM quinpirole in H2O). Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay. 5mL and 30mL sizes are also available.

Measure Gi signaling kinetics or activation with this live cell cAMP assay. This is a update to #X0200G, packaged in Big Sky BacMam providing a 3-4x increase in expression in cell types including HEK293 and CHO cells. BacMam also robustly transduces many iPSC and primary cultures, including neuronal and cardiac cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (D2R BacMam and 2mM quinpirole in H2O). Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay. 5mL and 15mL sizes are also available.

The original lipid-protein interaction assay and the trusted solution for the identification of protein-lipid interactions.  Mega Lipid Strips determine the binding specificity of lipid-binding proteins. Easy-to-use 2.5 x 6 cm membrane. Includes 23 major membrane lipids. 20+ years' experience proven & published. Cost effective 5-pack includes a complimentary vial of positive control.A simple and effective tool designed for the screening and analysis of protein-lipid interactions; Mega Lipid Strips combine our two most popular strips for 23 lipids on one complete strip. These lipids are found in cell membranes and interact with proteins to regulate cellular processes.Simple to use once the membrane is blocked and incubated with the protein of interest the membrane is washed. Then incubated with an HRP labeled secondary antibody. The membrane is then washed before detection with precipitating TMB or ECL reagent.Each batch of Mega Lipid Strips undergoes testing against a dive