Assays & Kits

The CUTANA™ ChIC/CUT&RUN Kit enables streamlined chromatin profiling of histone post-translational modifications (PTMs) and chromatin associated proteins. The CUT&RUN Kit Version 5 (v5) now includes an additional control antibody (H3K27me3). Positive (H3K4me3 and H3K27me3) and negative (IgG) control antibodies pair with SNAP-CUTANA™ spike-in controls for assay optimization and continuous assay monitoring (Figure 2). E. coli DNA is included for data normalization. SPRI magnetic beads are used for DNA purification, enabling seamless multi-channel pipetting throughout the workflow to maximize throughput and reproducibility. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 5,000 cells. The inclusion of controls, as well as compatibility with diverse target types, sample inputs, and low cell numbers, make t

The Bicinchoninic acid (BCA) assay is a stable, sensitive and highly compatible method for the determination of protein concentration. It is commonly used for quantifying the total amount of proteins after extraction. The detection mechanism of BCA assay involves two reations. The first reation is based on the biruet reaction, in which the protein chelates with copper (II) ion in an alkaline environment and reduces the copper (II) to copper (I), forming a blue complex. The second reaction is the chelation of BCA with the cuprous ion, resulting in an intense purple color. The purple colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion. The complex exhibits a strong linear absorbance at 562 nm with increasing protein concentrations.

TargetMol Protein A/G Immunomagnetic Beads enables Protein A/G to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein A/G Immunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein A/G Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol Protein A Immunomagnetic Beads enables Protein A to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein AImmunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein A Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol Protein G Immunomagnetic Beads enables Protein G to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein G Immunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein G Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol Protein L Immunomagnetic Beads enables Protein L to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein L Immunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein L Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol's HA Tag Immunomagnetic Beads specifically bind to proteins with the HA tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The HA Tag Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's MYC Tag Immunomagnetic Beads specifically bind to proteins with the MYC tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The MYC Tag Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's Flag Tag Immunomagnetic Beads specifically bind to proteins with the DYKDDDDK (Flag) tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Flag Tag Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.