Assays & Kits

The CD226:CD112 Homogeneous Assay Kit is designed to measure the inhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The CD226:CD155 Homogeneous Assay Kit is designed to measure the inhibition of CD226 binding to CD155 (PVR). The CD226:CD155 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD155, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is incubated with the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Please note this product may be subject to fees, we invite you to contact your local office. The CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-γ signaling. The key to this kit is the high sensitivity of detection of biotin-labeled SIRP-γ through streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-γ is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Please note this product may be subject to fees, we invite you to contact your local office. The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next, IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

The GITR:GITRL[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of GITR:GITRL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled GITR by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, GITRL is coated on a 96-well plate. Next, GITR is incubated with GITRL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

The CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-2 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-2 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

The CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of CTLA4 binding to B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

IDO1 Fluorescence Solution is an optimized, proprietary formulation containing necessary reaction components for BPS IDO1 Assay Kits. This product is intended to be used with the BPS Human IDO1 Fluorogenic Inhibitor Screening Assay Kit (BPS Cat. #72037 & Cat. #72047) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).

The ADAM17 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions. The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.