Assays & Kits

The fluorogenic Cathepsin V substrate is a Z-Leu-Arg-AMC dipeptide. It can be used as a substrate for activity assays of Cathepsins K, L, S, and V. λex 353-380 nm, λem=440-460 nm

The fluorogenic Cathepsin F substrate is a Z-Phe-Arg-AMC dipeptide. It can be used for the activity assays of Cathepsins B, F, and L. λex 353-380 nm, λem=440-460 nm

The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.

The PARP3 Chemiluminescent Activity Assay kit is designed to measure PARP3 activity for screening and profiling applications. The key to the PARP3 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with the assay buffer that contains the PARP3 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.

The PARP6 Chemiluminescent Activity Assay kit is designed to measure PARP6 activity for screening and profiling applications. The key to the PARP6 chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP6 reactions. First, histone proteins are coated on a microplate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP6 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The PARP10 Chemiluminescent Assay Kit is designed to measure PARP10 activity for screening and profiling applications. The key to the PARP10 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP10 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader

The PARP11 Chemiluminescent Activity Assay kit is designed to measure PARP11 activity for screening and profiling applications. The key to the PARP11 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP11 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The PARP15 Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. The key to the PARP15 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP15 reactions. First, histone proteins are coated onto the wells of the strip-plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP15 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The PARP14 Chemiluminescent Activity Assay Kit is designed to measure PARP14 activity for screening and profiling applications. The key to the PARP14 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP14 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP14 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader