Assays & Kits

The NNMT Fluorogenic Assay Kit is designed to measure NNMT (Nicotinamide N-methyltransferase) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant NNMT, substrate, cofactor, and assay buffer for 100 enzyme reactions.

The VEGFR1:VEGF165 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed for screening and profiling molecules that block the binding between VEGFR1 (vascular endothelial growth factor receptor 1, also known as fms related receptor tyrosine kinase 1) and VEGF165 (vascular endothelial growth factor 165). This kit comes in a convenient 96-well format, with enough recombinant biotin-labeled VEGF165 (amino acids 27-191(end)), purified VEGFR1 (amino acids 27-756), streptavidin-labeled HRP, and assay buffer for 100 binding reactions. A 96-well plate is coated with VEGFR1 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-VEGF165, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of VEGFR1 to VEGF165.

The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding is designed for the testing and profiling of Molecular Glues (MG) and PROTACs targeting CDK2 (cyclin dependent kinase 2) or CDK9 (cyclin dependent kinase 9) and Cereblon (CRBN). The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding comes in a convenient AlphaLISA® format, with enough PROTAC® buffer, purified CDK2/CyclinA2 and CDK9/CyclinK complexes, and CRBN for 384 reactions. This kit also contains the control PROTAC® (CDK2/9 Degrader) and CDK inhibitor FN-1501.The Molecular Glue/PROTAC® of interest is incubated with Cereblon (CRBN) and CDK2 or CDK9, bringing them into proximity. CRBN contains a FLAG-tag, which is recognized by anti-FLAG AlphaLISA„¢ acceptor bead. CDK contains a GST-tag that binds to the donor bead. Upon excitation of the donor bead, a singlet oxygen is generated by the bead. The singlet oxygen excites the acceptor bead, which emits light proportionally to the level of i

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (EC1.2.1.12) Glyceraldehyde-3-phosphoric acid catalyzed oxidation produce 1,3-diphosphate glyceric acid, is the key enzyme of glycolytic pathway, It is closely related to gluconeogenesis pathway, maintenance of blood glucose concentration in the body and the occurrence of diabetes, and plays an important role in the disorders of sugar, lipid and protein metabolism. CheKine™ Micro Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit can detect animal and plant tissues, cells or bacteria, culture solution or other liquid samples. In this kit, 3-phosphoglycerate kinase catalyzes the formation of 1,3-diphosphoglycerate from triphosphoglycerate and ATP. GAPDH reversely catalyzes 1,3 diphosphoglyceric acid and NADH to produce glyceraldehyde 3-phosphate, inorganic phosphorus and NAD+. The decrease of NADH measured at 340 nm can reflect the level of GAPDH activity.

Lipid hydroperoxide (LPO) is a peroxide produced by the action of unsaturated fatty acid chains by free radicals or reactive oxygen species. In pathological conditions, the enhancement of lipid peroxidation can lead to the increase of the originally low level of LPO. The increase of LPO content will cause damage to the structure and function of cells, and LPO content is closely related to the immune system and aging. CheKine™ Micro Lipid Peroxidation (LPO) Content Assay Kit can be used to detect biological samples such as animal and plant tissue, cells, plasma, serum or other liquid samples. In the kit, LPO is heated under acidic conditions to produce malondialdehyde (MDA), which condense with Thiobarbituricacid (TBA) to produce the brown-red substance trimethicine (3,5,5-trimethyloxazol-2,4-dione), whose maximum absorption wavelength is 532 nm. The content of LPO in the sample can be estimated by colorimetry.

L-galactose pathway is the main pathway of plant ascorbicacid (AsA) synthesis. L-galactose glucoside-1,4-lactone dehydrogenase (L-Galactono-1,4-Lactone Dehydrogenase, Gal LDH) is located in the mitochondrial membrane, responsible for the final step of the catalytic AsA biosynthesis in plants, is one of the key enzyme of the way. It plays a crucial role in the accumulation of AsA content in plants. CheKine™ Micro L-Galactono-1,4-Lactone Dehydrogenase (Gal LDH) Activity Assay Kit can detect plant tissues In this kit, Gal LDH catalyzed L-galactolactone reduction and oxidation of cytochrome C (Cyt c), and the reduced Cyt c had an absorption peak at 550 nm. Gal LDH activity was calculated by measuring the rate of increase of reduced Cyt c.

Ploygalacturonase (PG) (EC 3.2.1.15) is a kind of pectinase, which is widely found in plants, bacteria and fungi. It catalyses the decomposition of polygalacturonic acid, which plays an important role in fruit softening, pollen pollination, seed maturation and organ shedding. In addition, when pathogenic bacteria infect host plants, they can secrete polygalacturonase to degrade host cell wall, thus leading to the development of disease. CheKine™ Micro Ploygalacturonase (PG) Activity Assay Kit can be used to detect biological samples such as plant tissue, bacteria,fungus, liquid samples. In the kit, PG hydrolyzes polygalacturonic acid to produce galaturonate, which has a reducing aldehyde group, reacts with DNS reagent to produce reddish-brown substance, and has a characteristic absorption peak at 540 nm. The activity of polygalacturonase can be calculated by measuring the change of absorption value at 540 nm.

Trehalase (THL) (EC3.2.1.28) is widely found in animals, plants, microorganisms and cultured cells. The main function of trehalase is that the organism breaks down trehalose to produce glucose, which is directly used for energy supply. CheKine™ Micro Trehalase (THL) Activity Assay Kit can be used to detect biological samples such as animal and plant tissue, cells or fungus, plasma, serum or other liquid samples. In the kit, THL catalyzes trehalose to produce glucose, glucose oxidase catalyzes glucose oxidation to gluconic acid, and hydrogen peroxide. Peroxidase catalyzes hydrogen peroxide to oxidize 4-amino-antipyrine conjugated phenol to produce colored compounds with characteristic absorption peaks at 505 nm, reflectingTHL activity.