Cell-based Assays

L-galactose pathway is the main pathway of plant ascorbicacid (AsA) synthesis. L-galactose glucoside-1,4-lactone dehydrogenase (L-Galactono-1,4-Lactone Dehydrogenase, Gal LDH) is located in the mitochondrial membrane, responsible for the final step of the catalytic AsA biosynthesis in plants, is one of the key enzyme of the way. It plays a crucial role in the accumulation of AsA content in plants. CheKine™ Micro L-Galactono-1,4-Lactone Dehydrogenase (Gal LDH) Activity Assay Kit can detect plant tissues In this kit, Gal LDH catalyzed L-galactolactone reduction and oxidation of cytochrome C (Cyt c), and the reduced Cyt c had an absorption peak at 550 nm. Gal LDH activity was calculated by measuring the rate of increase of reduced Cyt c.

Ploygalacturonase (PG) (EC 3.2.1.15) is a kind of pectinase, which is widely found in plants, bacteria and fungi. It catalyses the decomposition of polygalacturonic acid, which plays an important role in fruit softening, pollen pollination, seed maturation and organ shedding. In addition, when pathogenic bacteria infect host plants, they can secrete polygalacturonase to degrade host cell wall, thus leading to the development of disease. CheKine™ Micro Ploygalacturonase (PG) Activity Assay Kit can be used to detect biological samples such as plant tissue, bacteria,fungus, liquid samples. In the kit, PG hydrolyzes polygalacturonic acid to produce galaturonate, which has a reducing aldehyde group, reacts with DNS reagent to produce reddish-brown substance, and has a characteristic absorption peak at 540 nm. The activity of polygalacturonase can be calculated by measuring the change of absorption value at 540 nm.

Trehalase (THL) (EC3.2.1.28) is widely found in animals, plants, microorganisms and cultured cells. The main function of trehalase is that the organism breaks down trehalose to produce glucose, which is directly used for energy supply. CheKine™ Micro Trehalase (THL) Activity Assay Kit can be used to detect biological samples such as animal and plant tissue, cells or fungus, plasma, serum or other liquid samples. In the kit, THL catalyzes trehalose to produce glucose, glucose oxidase catalyzes glucose oxidation to gluconic acid, and hydrogen peroxide. Peroxidase catalyzes hydrogen peroxide to oxidize 4-amino-antipyrine conjugated phenol to produce colored compounds with characteristic absorption peaks at 505 nm, reflectingTHL activity.

Pectinase is a general term for a class of enzymes that decompose pectin, including polygalacturonase, pectin esterase, pectin lyase, and pectin methylesterase. These enzymes are widely found in plant fruits and microorganisms and are primarily used in industries such as food, winemaking, environmental protection, pharmaceuticals, textiles, and daily chemical products. CheKine™ Mirco Pectinase Activity Assay Kit offers a simple, convenient, and rapid approach for assessing sucrase activity, which is suitable for plant tissues, bacteria, fungi, cell culture media or other Liquid samples. The principle of the kit is based on the hydrolysis of pectin by pectinase to produce galacturonic acid, which has a reducing aldehyde group. This reacts with DNS reagent to form a reddish-brown substance that has a characteristic absorbance peak at 540 nm. Measuring the changes in absorbance at 540 nm allows for the calculation of pectinase activity.

Pectin lyase (EC4.2.2.10) is an important component of pectinases, which is a depolymerase that can degrade plant cell walls,leading to the softening or even death of plant tissues. It has a wide range of sources, mainly from microorganisms. It can be used for clarification of fruit juice and wine, improving the yield of fruit juice, purification of plant viruses, bleaching of pulp, and biorefining of textiles. It has potential application value in reducing environmental pollution and lowering energy consumption. The CheKine™ Mirco Pectin Lyase Activity Assay Kit offers a simple, convenient, and rapid method for detecting PL activity, suitable for samples such as plant tissues, bacteria, fungi, and culture media. Its principle involves pectin lyase acting on the α-1,4-glycosidic bonds in pectin to produce unsaturated oligogalacturonic acids with an unsaturated bond between C4 and C5 at the reducing end, which have a characteristic absorption peak at 235 nm.

Pectin is a major component of the primary cell wall and middle lamella, primarily consisting of protopectin, pectic acid methylester, and pectic acid. Pectin contains galacturonic acid, galactose, arabinose, glucuronic acid, and others, making it one of the most abundant polysaccharides in the cell walls of many higher plants. Its unique physical and chemical properties affect the texture and quality of plant-derived foods. Pectins are cross-linked by Ca2+ bridges and other ionic bonds, hydrogen bonds, glycosidic bonds, ester bonds, and phenolic ring couplings. Various forms of pectin can be extracted through different extraction methods, such as water-soluble pectin (WSP), ionically-bound pectin (ISP), and covalently-bound pectin (CSP). CheKine™ Mirco Soluble Pectin (WSP) Content Assay Kit provides a simple, convenient, and rapid method for WSP content determination suitable for plant tissue samples. The principle involves using an acidic solution to extract soluble pectin (WSP), fol

The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix. CheKine™ Micro Plant Root Vitality (TTC-Method) Assay Kit can be used to detect biological samples such as plant tissues. In this kit, The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenyl formazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorption peak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.

Sucrose is not only an important photosynthetic product but also the primary substance transported within plants and serves as a form of carbohydrate storage. Sucrose phosphate synthase (SPS, EC 2.4.1.14) uses fructose-6-phosphate as a receptor, and the formed sucrose-6-phosphate is converted into sucrose by sucrose phosphate phosphatase. The SPS-sucrose phosphate phosphatase system is generally considered the main pathway for sucrose synthesis. CheKine™ Mirco Sucrose Phosphate Synthase (SPS) Activity Assay Kit provides a simple, convenient, and rapid method for detecting SPS activity, suitable for plant tissue samples. The principle involves SPS catalyzing the formation of sucrose-6-phosphate from fructose-6-phosphate. Sucrose-6-phosphate reacts with resorcinol to produce a color change, exhibiting a characteristic absorption peak at 480 nm, with the intensity of the color being proportional to the level of enzyme activity.

Sucrose is the primary form in which photosynthetic products from sources (such as leaves) are transported to “sink” organs. Sucrose synthase (EC 2.4.1.13) is a reversible enzyme that can catalyze both the synthesis and breakdown of sucrose, making it a key enzyme in sucrose metabolism. Studying the activity of its invertase direction (SS-I) is significant for understanding plant sucrose degradation and starch synthesis. CheKine™ Mirco Sucrose Synthase-I (SS-I) Activity Assay Kit offers a simple, convenient, and rapid approach for detecting sucrose synthase (SS-I) activity, suitable for plant tissue samples. The principle of the kit involves SS-I catalyzing the reaction between sucrose and UDP to produce free fructose and UDPG; the content of reducing sugars is then measured using the 3,5-dinitrosalicylic acid method to reflect the level of enzyme activity