Cell-based Assays

Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels

Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels

Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels

Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels

THUNDER Phosphatase Inhibitor Cocktail (100X) is specially formulated for use with Phospho and Total THUNDER Cell Signaling Assay Kits. This cocktail is already provided in these kits, but can be ordered separately.

THUNDER Phosphatase Inhibitor Cocktail (100X) is specially formulated for use with Phospho and Total THUNDER Cell Signaling Assay Kits. This cocktail is already provided in these kits, but can be ordered separately.

THUNDER Phosphatase Inhibitor Cocktail (100X) is specially formulated for use with Phospho and Total THUNDER Cell Signaling Assay Kits. This cocktail is already provided in these kits, but can be ordered separately.

Measure 3CL protease (3CLpro) inhibition or screen for inhibitors with the live cell, fluorescent 3CLglow assay. The 3CLglow sensor expresses a green fluorescent protein in the presences of the 3CLpro enzyme as well as a constitutively expressed red fluorescent protein to monitor cell health and compound toxicity. The assay is packaged in BacMam for expression in mammalian cells. BacMam provides even, near 100% transduction efficiency in HEK293 cells as well as expression in many other cell lines, iPSCs, and primary cultures including A549s and HUVECs. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity. This kit includes the 3CLglow sensor BacMam, 3CLpro enyme BacMam, and 500mM sodium butyrate in H2O. 5mL and 60mL sizes are also available.

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing the highly water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit- 8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.