Functional Assays

SucrosePhosphorylase (SP, EC2.4.1.7) is mainly present in microorganisms and plants and belongs to the glycosylhydrolase 13 family. It is an enzyme that catalyzes the transfer of glucoside bonds and can catalyze the synthesis of 1-phospho-glucose from sucrose and inorganic phosphate. This enzyme mainly uses sucrose and glucose 1-phosphate as donors, and many substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyl groups, carboxyl groups as receptors to catalyze the synthesis of various glycosides. CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit can detect plant tissues, fungus samples. In this kit, SP can catalyze sucrose to produce glucose 1-phosphate, which is modified to glucose 6-phosphate under the catalysis of glucose phosphomutase, and reduce NADP+ to generate NADPH under the action of glucose 6-phosphate dehydrogenase, resulting in an increase in the light absorption value of 340nm. The SP activity was reflected by the increase rate of 340nm abs

used to detect triglyceridel levels

used to detect cholestrol levels

used to detect Phospholipid levels

The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure METTL3/METTL14 complex activity for screening and profiling applications. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6-methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

The ADAR1:RNA TR-FRET Assay Kit is designed to measure the binding of ADAR1 (adenosine deaminase, RNA-specific 1) to RNA for screening and profiling applications using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). It utilizes Terbium-labeled donor and dye-labeled acceptor to complete the TR-FRET pairing. The ADAR1:RNA TR-FRET Assay Kit comes in a convenient 384-well format, with enough purified ADAR1, Tb-Labeled Donor and Dye-Labeled Acceptor, RNase inhibitor, ADAR1 substrate and assay buffer for 384 reactions. The assay also includes a Competitor RNA as control.

Vγ9Vδ2 T Cell Expansion Kit is suitable for the ex vivo culture and expansion of human Vγ9Vδ2 T cells. γδ T cells are low abundance in PBMCs (< 2%) and therefore they need to be successfully activated and expanded to obtain adequate number of cells for γδ T-cell based immunotherapy related studies. This kit contains media and reagents necessary to drive the robust activation and expansion of the Vγ9Vδ2 T cells subpopulation from PBMCs. To improve the purity of the expanded cells, αβ T cells and B cells are depleted from the expansion culture using an antibody cocktail. This kit is provided with enough reagents and materials for the activation and expansion of Vγ9Vδ2 T cells from a starter population of 10 x 10⁷ PBMCs.

Triose-phosphateisomerase (TPI) in plant chloroplasts is an important enzyme involved in calvin cycle in photosynthesis. Acting on the transformation between glyceraldehyde phosphate and dihydroxyacetone phosphate, dihydroxyacetone phosphate can quickly penetrate the chloroplast envelope and enter the cytoplasm, where it is gradually transformed into sucrose. CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit can detect plant tissues In this kit, TPI converts dihydroxyacetone phosphate into glyceraldehyde-3-phosphate, and glyceraldehyde-3-phosphate and NAD react with glyceraldehyde-3-phosphate dehydrogenase to generate glyceric acid-3-phosphate and NADH. The absorbance change at 340 nm reflects the activity of TPI.

β-Xylosidase (EC 3.2.1.37) exists in organisms such as plants, bacteria, and fungi. It is a key enzyme that catalyzes the degradation of xylan-based hemicelluloses, and the product xylose can serve as a carbon source for microbial fermentation. Additionally, β-xylosidase can be used as a biobleaching agent in the paper industry, offering environmental benefits over traditional bleaching methods and possessing broad application value. CheKine™ Mirco β-Xylosidase (β-X) Activity Assay Kit offers a simple, convenient, and rapid approach for assessing β-Xylosidase activity, which is suitable for plant tissue, bacteria, and fungi samples. The principle involves β-xylosidase catalyzing the production of p-nitrophenol from p-nitrophenyl-β-D-xyloside. p-Nitrophenol exhibits a characteristic absorption peak at 405 nm. By measuring the rate of increase in absorbance at 405 nm, the β-xylosidase activity can be calculated.