Functional Assays

Fructose -1,6- diphosphatase (FBP), also known as fructose -1,6- diphosphatase, plays a key role in gluconeogenesis and the synthesis of sucrose, an assimilate of photosynthesis. Catalyze fructose-1,6- diphosphate and water to produce fructose-6- phosphate and inorganic phosphorus. CheKine™ Micro Fructose 1,6-Bisphosphatase (FBP) Activity Assay Kit can detect animal and plant tissues, cells, plasma, serum or other liquid samples. In this kit, FBP catalyzes fructose-1,6-diphosphate and water toproduce fructose-6-phosphate and inorganic phosphorus. Glucose-6-phosphate isomerase and glucose-6-phosphate dehydrogenase added in the reaction system catalyze glucose-6-phosphate and NADPH in turn, and the activity of FBP can becalculated by measuring the increase rate of NADPH at 340 nm.

Fructose 1,6-diphosphate aldolase (FBA)(EC.4.1.2.13) is an important enzyme involved in calvin cycle in glycolysis, gluconeogenesis, pentose phosphate pathway and photosynthesis, which catalyzes the reversible cleavage of fructose 1,6-diphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, and is widely used. CheKine™ Micro Fructose 1,6-Bisphosphate Aldolase (FBA) Activity Assay Kit can detect animal and plant tissues, cells, fungus, plasma, serum or other liquid samples. In this kit, FBA catalyzes fructose-1,6-diphosphate to produce glyceraldehyde-3-phosphate and dihydroxyacetone-3-phosphate, and NADH and dihydroxyacetone-3-phosphate are catalyzed to produce NAD and α-glycerophosphate under the action of triose phosphate isomerase and α-glycerophosphate dehydrogenase. The change of absorbance at 340 nm can reflect the activity of FBA.

Trehalose is widely found in animals, plants, microorganisms and cultured cells. Because trehalose has unique biological characteristics different from other carbohydrates, it can protect the protein, fat, sugar, nucleic acid and other components of biological cells from damage under the harsh environment such as drought, high temperature, dehydration, freezing, high osmotic pressure and toxic substances. CheKine™ Micro Trehalose Content Assay Kit can be used to detect biological samples such as animal and plant tissue, cells or bacteria, plasma, serum or other liquid samples. In the kit, the method was determined by anthrone colorimetric method. It has the advantages of high sensitivity, simple and fast, and is suitable for the determination of trace samples. However, anthrone colorimetric method also has some defects, if the sample contains soluble sugar, it will affect the determination. This kit is recommended for the determination of samples that do not contain soluble sugars othe

Pectin is one of the main components of plant cell wall, which is divided into water-soluble pectin and insoluble pectin, namely protopectin. It is widely used in food, textile, printing and dyeing, tobacco, metallurgy and other fields because of its good emulsification, thickening and gelation. CheKine™ Micro Protopectin Content Assay Kit can be used to detect biological samples such as plant tissue. In the kit, protopectin was hydrolyzed into soluble pectin in dilute acid, and further converted into galacturonic acid. The product was condensed with carbazole in strong acid to form a purplish red compound with characteristic absorption at 530 nm.

Hemicellulose is a mixture of polysaccharides that is tightly bound to cellulose in plant cell walls and is a major component of the primary cell wall. It is widely present in plants and represents a novel exploitable energy source. CheKine™ Mirco Hemicellulose Concent Assay Kit offers a simple, convenient, and rapid approach for assessing hemicellulose concent, which is suitable for plant tissue samples. The principle involves the conversion of hemicellulose into reducing sugars through acid hydrolysis. These reducing sugars then react with DNS (3,5-dinitrosalicylic acid) to form a reddish-brown compound that exhibits a characteristic absorption peak at 540 nm. The magnitude of the absorbance value is indicative of the amount of hemicellulose present.

Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, and is one of the key enzymes in sucrose metabolism in higher plants. According to the optimal pH, higher plant Ivr can be divided into acidic invertase (AI) and Neutralinvertase (NI) types. The optimal pH of AI is 3~5. There are two types of AI: soluble AI(S-AI) and cell wall insoluble AI(B-AI). S-AI mainly exists in cellular vacuoles or free space, and the optimal pH is 4.5~5.0 (acidic). By degrading sucrose in vacuoles, S-AI regulates the utilization of sucrose in vacuoles and the accumulation of sugars in fruits. CheKine™ Micro Soluble Acid Invertase (S-AI) Activity Assay Kit can detect plant tissues samples. In this kit, S-AI catalyzes sucrose degradation to produce reducing sugar, which further reacts with 3,5-dinitrosalicylic acid to produce brown-red amino compounds with characteristic light absorption at 510 nm, and the rate of increase of light absorption at 510 nm is proportional t

Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, and is one of the key enzymes in sucrose metabolism in higher plants. According to the optimal pH, higher plant Ivr can be divided into acidic invertase (AI) and Neutralinvertase (NI) types. The optimal pH of AI is 3~5. There are two types of AI: soluble AI(S-AI) and cell wall insoluble AI(B-AI). B-AI exists in the intercellular space and binds to the cell wall, and is mainly involved in sucrose decomposition during extracellular unloading in phloem to maintain the concentration of sucrose between pool sources. CheKine™ Micro Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit can detect plant tissues samples. In this kit, B-AI catalyzes sucrose degradation to produce reducing sugar, which further reacts with 3,5-dinitrosalicylic acid to produce brown-red amino compounds with characteristic light absorption at 510 nm, and the rate of increase of light absorption at 510 nm is proportion

METTL3 (methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit)/METTL4 (methyltransferase 4, N6-adenosine) and METTL14 (methyltransferase 14, N6-adenosine) form the core of a complex involved in N6-methyladenosine addition to RNA, one of the most common mRNA modifications, and belong to the group known as "writer" proteins. METTL3 expression is altered in cancer via different processes. For instance, in pancreatic cancer cigarette smoke condensate influences METTL3 promoter hypomethylation and increases its expression. This complex can act as a tumor suppressor or activator and promote chemoresistance.  It has been linked to acute myeloid leukemia (AML), liver, lung, bladder cancer and others. Interestingly METTL3 can activate certain oncogenes by simply recruiting eIF3 (eukaryotic translation initiation factor 3), a process that does not require its catalytic activity. Its role in cancer has made it an attractive target in oncology. STM2457, a highly selective p

The NRG1β: HER3 (ERBB3) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between NRG1β (neuregulin-1β) and HER3 (human epidermal growth factor receptor 3, also known as ERBB3) for screening and profiling applications. The NRG1β: HER3 (ERBB3) Chemiluminescent Assay Kit comes with enough purified NRG1β (amino acids 2-246) and HER3 (amino acids 20-643) proteins, detection antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with NRG1β protein. After coating and blocking, HER3 is added in an optimized assay buffer. Unbound HER3 is washed away, and the plate is incubated with a detection antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of HER3 binding to NRG1β.