Functional Assays

The NRG1β: HER4 (ERBB4) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between NRG1β (neuregulin-1β) and HER4 (human epidermal growth factor receptor 4, also known as ERBB4) for screening and profiling applications. The NRG1β: HER4 (ERBB4) Chemiluminescent Assay Kit comes with enough purified NRG1β (amino acids 2-246) and HER4 (amino acids 26-651) proteins, detection antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with NRG1β protein. After coating and blocking, HER4 is added in an optimized assay buffer. Unbound HER4 is washed away, and the plate is incubated with a detection antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of HER4 binding to NRG1β.

The NNMT Fluorogenic Assay Kit is designed to measure NNMT (Nicotinamide N-methyltransferase) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant NNMT, substrate, cofactor, and assay buffer for 100 enzyme reactions.

The VEGFR1:VEGF165 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed for screening and profiling molecules that block the binding between VEGFR1 (vascular endothelial growth factor receptor 1, also known as fms related receptor tyrosine kinase 1) and VEGF165 (vascular endothelial growth factor 165). This kit comes in a convenient 96-well format, with enough recombinant biotin-labeled VEGF165 (amino acids 27-191(end)), purified VEGFR1 (amino acids 27-756), streptavidin-labeled HRP, and assay buffer for 100 binding reactions. A 96-well plate is coated with VEGFR1 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-VEGF165, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of VEGFR1 to VEGF165.

The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding is designed for the testing and profiling of Molecular Glues (MG) and PROTACs targeting CDK2 (cyclin dependent kinase 2) or CDK9 (cyclin dependent kinase 9) and Cereblon (CRBN). The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding comes in a convenient AlphaLISA® format, with enough PROTAC® buffer, purified CDK2/CyclinA2 and CDK9/CyclinK complexes, and CRBN for 384 reactions. This kit also contains the control PROTAC® (CDK2/9 Degrader) and CDK inhibitor FN-1501.The Molecular Glue/PROTAC® of interest is incubated with Cereblon (CRBN) and CDK2 or CDK9, bringing them into proximity. CRBN contains a FLAG-tag, which is recognized by anti-FLAG AlphaLISA„¢ acceptor bead. CDK contains a GST-tag that binds to the donor bead. Upon excitation of the donor bead, a singlet oxygen is generated by the bead. The singlet oxygen excites the acceptor bead, which emits light proportionally to the level of i

All-in-one kit for making phase-separated droplets with BSA (Bovine Serum Albumin), Droplets are easy to observe using only a micropipette and microscope, Includes a user's manual with calculation examples and details on how to use it

Set of reagents necessary to confirm the conditions for making phase-separated droplets, Crowding agent, pH, and salt can be optimized with this kit, Includes user's manual with sample calculations and methods for adjusting buffer pH

The iQuant™ microRNA Assay Kit is ideal for quantification of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay kit is highly selective for small RNA over rRNA or large mRNA. The assay kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The assay kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted microRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

The TR-FRET In vitro E3 activity kit has been developed to be simple, fast, and easy to use while being robust and sensitive. As E3 ligases autoubiquitinate they build long polyubiquitin chains; our unique assay will detect these chains as they are being built. This assay is homogenous and compatible for High-throughput Screening (HTS). Choose 1 E2 option among: UBE2D3 (UbcH5c), UBE2L3 (UbcH7), UBE2K (UbcH1), Ubc13, Mms2 (yeast), UBE2D2 (UbcH5b, E2-17K2), UBE2R1 (CDC34), UBE2R2 (Ubc3B), Non-tagged UBE2D1, His6-SUMO tagged UBE2D1, UBE2E2 (UbcH8), UBE2E3 (UbcH9), His6-tagged UBE2H (E2-20K), His6-SUMO tagged UBE2H (E2-20K), UBE2F (NCE2), UBE2M (Ubc12), UBE2N (Ubc13), Non-tagged UBE2T (PIG50), His6-SUMO tagged UBE2T (PIG50), UBE2V2 (MMS2, UEV-2), UBE2S (E2EPF), UBE2C (Ubc10), His6-tagged UBE2W (Ubc 16), UBE2Q2, His6-tagged UBE2A, Non-tagged UBE2G2 (Ubc7), His6-SUMO tagged UBE2G2 (Ubc7), His6-tagged UBE2I (Ubc9), UBE2L6 (UbcH8), UBE2Z (Use1) And Choose 1 E3 option among: ITCH, Traf6, GP78,