Functional Assays

The Fc (IgG1): FcRn Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling neutralizing antibodies or inhibitors of the interaction between Fc (IgG1) and human FcRn.  The assay requires only a few steps. First, Fc (IgG1) is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-FcRn, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN inhibitor NCGC00063279 is also included as a control. The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.