Functional Assays

The TNKS1 (also known as PARP5a) Homogeneous Assay Kit is designed to measure TNKS1 activity for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS1 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, a sample containing TNKS1 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The TNKS2 (also known as PARP5b) Homogeneous Assay Kit is designed to measure TNKS2 activity for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to its substrate. The key to the TNKS2 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, a sample containing TNKS2 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The PARP3 Homogeneous Assay Kit is designed to measure PARP3 activity for screening and profiling applications. PARP3 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP3 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, a sample containing PARP3 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

The PARP11 Homogeneous Assay Kit is designed to measure PARP11 activity for screening and profiling applications. PARP11 is known to catalyze the NAD-dependent addition of ADP-ribose to its substrates. The key to the PARP11 Homogeneous Assay Kit is a highly specific antibody that recognizes PARylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, a sample containing PARP11 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Ethanol Assay Kit is a colorimetric determination of ethanol at 565 nm.

Biotin Assay Kit is a quantitative colorimetric determination of biotin in food, cosmetic, and biotinylated proteins at 500 nm.

Boron Assay Kit is a quantitative colorimetric determination of boron in agricultural and environmental samples at 420 nm.

Cholesterol Uptake Assay Kit is a quantitative fluorometric determination of cholesterol uptake in adherent cells.

Detergent Assay Kit is a quantitative colorimetric determination of detergent in purified protein samples, water and soil at 560 nm or 650 nm.