Functional Assays

The Tie2 Kinase Assay Kit is designed to measure Tie2 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

The TNKS1 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 1 (TNKS1, also known as PARP5A) for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS1 (PARP5A) Colorimetric Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS1 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.

The TNKS2 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Colorimetric Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the Colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.

The IL-2: IL-2 Receptor Alpha (IL-2RA) Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of inhibitors or neutralizing antibodies blocking the interaction between human IL-2 and its receptor IL-2RA.  The assay requires only a few steps. First, IL-2RA is coated on a 96-well plate overnight. After blocking, the receptor is pre-incubated with the test inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-IL-2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

RayBio® Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts. Dry ice shipment (additional fee). 2 - 4 week lead time.

RayBio® Human GATA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-2 binding sequences to semi-quantitatively detect active GATA-2 in lysates or nuclear extracts. Dry ice shipment (additional fee). 2 - 4 week lead time.

RayBio® Human GATA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-2 binding sequences to semi-quantitatively detect active GATA-2 in lysates or nuclear extracts. Dry ice shipment (additional fee). 2 - 4 week lead time.

RayBio® Human GATA-3 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-3 binding sequences to semi-quantitatively detect active GATA-3 in lysates or nuclear extracts. Dry ice shipment (additional fee). 2 - 4 week lead time.

The ChoosE3-Freedom Intrachain TR-FRET Assay Kit contains E1 and E2 enzymes, ATP, an optimized TRF Ubiquitin Mix, and a universal buffer. Purified E3 ligase MDM2 is also provided as an internal quality control. The assay was designed with a Europium-labeled Ubiquitin donor and a Cy5-labeled Ubiquitin acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on the E3 ligase, the assay measures only poly-ubiquitination and not mono-ubiquitination. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics.