Functional Assays

The BTK (C481S) Kinase Assay Kit is designed to measure BTK (C481S) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

The HSP90β (C-terminal domain) TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure the inhibition of HSP90β binding to its target protein PPDI in a homogeneous 96-well format or 384-well format. It utilizes a Terbium-labeled donor and a Dye-labeled acceptor to complete the FRET pairing. This TR-FRET based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The Terbium-labeled donor will bind to PPDI, while the Dye-labeled acceptor will bind to HSP90β. When PPID is bound to HSP90β TR-FRET occurs and it can be measured using a fluorescence plate reader.

The HSP90β (C-terminal domain) TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure the inhibition of HSP90β binding to its target protein PPDI in a homogeneous 96-well format or 384-well format. It utilizes a Terbium-labeled donor and a Dye-labeled acceptor to complete the FRET pairing. This TR-FRET based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The Terbium-labeled donor will bind to PPDI, while the Dye-labeled acceptor will bind to HSP90β. When PPID is bound to HSP90β TR-FRET occurs and it can be measured using a fluorescence plate reader.

The 3CL Protease (T21I, E166V) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of T21I, E166V mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. The kit contains enough purified 3CL Protease (T21I, E166V) (BPS Bioscience #101671), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the subst

The 3CL Protease (T21I, A173V, T304I) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of T21I, A173V, T304I mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. The kit contains enough purified 3CL Protease (T21I, A173V, T304I) (BPS Bioscience #101679), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More inf

The 3CL Protease (T21I, A173V) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of 3CL Protease mutated at T21I and A173V for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).

The 3CL Protease (T21I, T304I) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of T21I and T304I mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. The kit contains enough purified 3CL Protease (T21I, T304I) (BPS Bioscience #101685), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the

The breast tumor kinase (BRK) Kinase Assay Kit is designed to measure BRK tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. 

The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions. The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.