Functional Assays

The Chemi-Verse™ CDK16/Cyclin Y Kinase Assay is a luminescent assay designed to measure CDK16 (cyclin-dependent kinase 16)/Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK16 (amino acids 107-496)/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ CDK18/Cyclin Y Kinase Assay is a luminescent assay designed to measure CDK18 (cyclin-dependent kinase 18)/Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK18/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ CDK14/Cyclin Y Kinase Assay Kit is a luminescent assay designed to measure CDK14 (cyclin-dependent kinase 14)/Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK14/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The JAK1 (JH2 Pseudokinase Domain) Inhibitor Screening Assay Kit is a fluorescence polarization-based assay designed for screening and profiling small molecules that displace the fluorescently labeled probe (JH2 probe 1) from the JH2 pseudokinase domain of JAK1. This kit comes in a convenient 384-well format, with enough recombinant human JAK1 (JH2 Pseudokinase Domain), buffer, and fluorescently labeled JH2 probe 1 for 384 reactions. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) to read the FP signal. For more information on the principles of FP, visit bpsbioscience.com/pub/media/ebook_pdf/f/p/fp_assays.pdf. The assay is based on the competition between the test compound and the JH2 probe for the purified JAK1 (JH2 Pseudokinase Domain). JH2 probe 1 is incubated with JAK1 (JH2 Pseudokinase Domain) in the presence or absence of inhibitor. When the JH2 probe 1 is bound to the JH2 pseudokinase domain it has high FP due to its restri

The Dcaf11-driven p21 ubiquitination assay kit is a sensitive AlphaLisa® high-throughput screening (HTS) assay kit, designed to measure Dcaf11 (DDB1- and Cul4-associated factor 11) E3 ligase activity in a homogeneous 384 reaction format. The assay kit comes with enough biotinylated ubiquitin, ATP, GST-tagged p21, assay buffer, detection buffer, purified UBE1 (E1), UbcH5b (E2), and Rbx1/Cul4B/Dcaf11/Ddb1 complex (E3) for 384 reactions. The assay detects mono-ubiquitination and poly-ubiquitination of p21. First, E1 and E2 enzymes are incubated with the E3 complex and with the target protein p21 (GST-tag) in the presence of biotin-conjugated ubiquitin and ATP. Ubiquitination of p21 occurs in a multistep ubiquitin transfer from E1 to E2 to E3, and E3-mediated conjugation of ubiquitin to p21. Next, glutathione-acceptor beads are added, followed by streptavidin-conjugated donor beads, and Alpha-counts are measured. The increase in Alpha-counts is proportional to the mono- or poly-ubiquitinat

The DUB-Freedom™ Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of several purified deubiquitinating (DUB) enzymes of interest in a homogeneous 96-reaction format and is ideal for screening and profiling applications. The kit contains enough Ubiquitinated-AMC substrate and assay buffer for 100 reactions. It also includes the purified catalytic domain of the DUB enzyme USP2 (ubiquitin carboxyl-terminal hydrolase 2) (amino acids 259-end) as a positive control and Ub-Aldehyde as control inhibitor. The enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using the purified catalytic domain of human USP2 protein and Ub-Aldehyde, a potent inhibitor of the DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC (Ub-AMC) is a flu

The Chemi-Verse™ ERK2 Kinase Assay Kit is a luminescence assay designed to measure ERK2 (extracellular signal regulated kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ERK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ Aurora Kinase B Assay Kit is a luminescence assay designed to measure Aurora Kinase B kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase B, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ Aurora Kinase A Assay Kit is a luminescence assay designed to measure Aurora Kinase A activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase A, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.