Functional Assays

The Chemi-Verse™ Aurora Kinase A Assay Kit is a luminescence assay designed to measure Aurora Kinase A activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase A, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ Aurora Kinase C Assay Kit is a luminescence assay designed to measure Aurora Kinase C activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ MEK1 Kinase Assay Kit is a luminescent assay designed to measure MEK1 (dual specificity mitogen-activated protein kinase kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant MEK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

This kit is used for the fast quantification of Trastuzumab/Herceptin® (Monkey) in serum,plasma and cell supernatant samples. (Homogenous Assays, no wash step, quantitative assay)

The DNA Polymerase θ Assay Kit is a fluorogenic assay designed to measure DNA Polymerase θ activity for screening and profiling applications, utilizing the fluorescent nucleic acid dye GroovyGreen™ for the quantitation of double-stranded DNA (dsDNA) products. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase θ enzyme (amino acids 1792-2590), DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.  DNA Polymerase θ uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecules (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.  

A kinetic biosensor assay for arrestin recruitment converts conformational changes in arrestin-3 (β-arrestin-2) to changes in fluorescence intensity. This kit detects arrestin recruitment at the unmodified µ-Opioid Receptor (MOR). The Borealis Arrestin Sensor, and MOR, are packaged in a BacMam viral vector for easy delivery to most cell types. Validated for use on fluorescence imaging systems and automated fluorescence plate readers.

Sircol 2.0 is the newest member of Biocolor's family of collagen kits, designed to simplify quantative measurement of soluble collagens from in-vivo & in-vitro samples. It provides enhanced sensitivity and specificity for accurate collagen measurement, surpassing the performance of our previous soluble collagen kit.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.