Functional Assays

The PARG Fluorogenic Assay Kit is a high-throughput, homogeneous 96-well or 384-well assay designed to measure the hydrolase activity of Poly (ADP-ribose) glycohydrolase (PARG) for screening and profiling applications, using a simple and straightforward fluorogenic assay. The PARG Fluorogenic Assay Kit contains enough purified recombinant PARG enzyme, substrate, and assay buffer for 100 or 400 enzyme reactions. <p style="text-align: center;"><img src="{{media url="wysiwyg/PARPs/78858.png"}}" alt="" width="388" height="188" /> Figure 1: Illustration of the assay principle. PARG is incubated with a fluorogenic ADP-ribose substrate in which the fluorophore is quenched by the presence of the ribose. PARG-mediated hydrolysis of the substrate between the ribose and the fluorochrome releases fluorescence that can be detected at λ=502 nm (excitation at λ=385 nm). Fluorescence intensity is directly proportional to PARG hydrolase activity. 

The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease. 

The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease. 

The Cathepsin V Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin V for screening and profiling applications. The Cathepsin V assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin V (amino acids 18-334), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The Cathepsin V Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin V for screening and profiling applications. The Cathepsin V assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin V (amino acids 18-334), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.  

The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.