Results for Functional Assays ( 10047 )
- From: £1,983.00
The MDM2-Driven p53 Ubiquitination Assay kit is a sensitive AlphaLisa® high-throughput screening (HTS) assay designed to measure MDM2 (mouse double minute 2 homolog) E3 ligase activity in a homogeneous 384 reaction format. The assay kit comes with enough biotinylated ubiquitin, ATP, FLAG-tagged p53, assay buffer, detection buffer, purified UBE1 (E1), UbcH5b (E2), and MDM2 (E3) for 384 reactions. The assay can detect mono-ubiquitination and poly-ubiquitination of p53. E1 and E2 enzymes are incubated with the E3 complex and FLAG-tagged p53, in the presence of biotin-conjugated ubiquitin and ATP. Ubiquitination of p53 occurs in a multistep ubiquitin transfer from E1 to E2 to E3, and E3-mediated conjugation of ubiquitin to p53. Next, acceptor beads are added, followed by streptavidin-conjugated donor beads. Alpha-counts are then measured. The increase in Alpha-counts is proportional to the mono- or poly-ubiquitination of the FLAG-tagged p53.
- From: £607.00
The Chemi-Verse™ PAK4 Kinase Assay Kit is designed to measure PAK4 (p21-activated kinase 4) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK4 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- From: £607.00
The Chemi-Verse™ PAK6 Kinase Assay Kit is designed to measure PAK6 (p21-activated kinase 6) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK6 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- From: £607.00
The Chemi-Verse™ PAK7 Kinase Assay Kit is designed to measure PAK7 (p21-activated kinase 7) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK7 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- From: £343.00
Von Hippel-Lindau, or VHL, is an E3 Ligase that mediates ubiquitination and proteasomal degradation of target proteins. VHL is indicated to play a large role in tumor suppression, particularly due to its interactions with HIF (hypoxia inducible factor). The VHL is typically used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling. VHL complex is a multi-subunit ubiquitin ligase constituted with VHL, Elongin B, Elongin C, Cullin-2 and Rbx1. Elongin B (ELOB) and Elongin C (ELOC) as a heterodimer binds to the BC-box motif present in SOCS- & VHL-box protein families. VHL is the substrate recognition component that brings target specificity. VHL is linked to scaffolding proteins Cullin-2/Rbx1 of the complex via adaptor protein Elongin C with Elongin B stabilizing the complex.
- From: £343.00
Von Hippel-Lindau, or VHL, is an E3 Ligase that mediates ubiquitination and degradation of target proteins. VHL is seen to play a large role in tumor suppression, particularly due to its interactions with HIF. VHL can be used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling.