Functional Assays

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL17 (C-C motif chemokine 17; CC chemokine TARC) in cell culture supernatants. The kit does not include a recombinant human CCL17/TARC standard, which must be purchased separately from R&D Systems® (catalog number 364-DN).

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL17 (C-C motif chemokine 17; CC chemokine TARC) in cell culture supernatants. The kit does not include a recombinant human CCL17/TARC standard, which must be purchased separately from R&D Systems® (catalog number 364-DN).

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL17 (C-C motif chemokine 17; CC chemokine TARC) in cell culture supernatants. The kit does not include a recombinant human CCL17/TARC standard, which must be purchased separately from R&D Systems® (catalog number 364-DN).

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL17 (C-C motif chemokine 17; CC chemokine TARC) in cell culture supernatants. The kit does not include a recombinant human CCL17/TARC standard, which must be purchased separately from R&D Systems® (catalog number 364-DN).

The DR3:TL1A Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the interaction between DR3 (Death Receptor 3) and TL1A (TNF-like ligand 1A). This kit comes in a convenient 96-well or 384-well format, with biotin-labeled TL1A, purified DR3, streptavidin-labeled HRP, and assay buffer for 100 or 400 binding reactions.

The DR3:TL1A Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the interaction between DR3 (Death Receptor 3) and TL1A (TNF-like ligand 1A). This kit comes in a convenient 96-well or 384-well format, with biotin-labeled TL1A, purified DR3, streptavidin-labeled HRP, and assay buffer for 100 or 400 binding reactions.

The ACHE Colorimetric Assay Kit is designed to measure the activity of ACHE (acetylcholinesterase) for screening and profiling applications. The ACHE colorimetric reaction is based on a modified Ellman's method, using the alternative substrate acetylthiocholine iodide (ATCI) and 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) to quantify amount of thiocholine produced from the hydrolysis of ATCI by ACHE. This assay kit comes in a convenient 96-well format, with purified ACHE enzyme (amino acids 32-614), ATCI, DTNB and assay buffer for 100 enzyme reactions. The absorption intensity of DTNB adduct (λ=410 nm) is used to measure the amount of thiocholine product generated, which is proportional to ACHE activity.

The Chemi-Verse™ ERK1 Kinase Assay Kit is designed to measure ERK1 (extracellular signal-regulated kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ERK1, MBP, ATP, and kinase assay buffer for 100 enzyme reactions.

The Notch1:DLL4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding of Notch1 (neurogenic locus notch homolog protein 1) and DLL4 (delta like canonical notch ligand 4). This kit comes in a convenient 96-well format, with enough recombinant purified Notch1, biotin-labeled DLL4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled DLL4 by streptavidin-HRP. Notch1 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-DLL4, the plate is washed and Streptavidin-HRP is added. After the ELISA ECL substrate is added the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of Notch1 to DLL4.