Cell Line

Please note this product may be subject to fees, we invite you to contact your local office. BCMA Knockout RPMI-8226 Cell Line is an RPMI-8226 cell line in which BCMA (B-Cell Maturation Antigen, or CD269) has been genetically removed from RPMi-8226 cells using CRISPR/Cas9 genome editing by RNP (ribonucleoprotein) electroporation. This cell line has been validated by genome sequencing and flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. The FcRn: IgG Recycling HMEC-1 Cell Pool is a HMEC-1 cell pool expressing Fc gamma receptor and transporter (FCGRT) (NM_004107.5) and beta-2-microglobulin (B2M) (NM_004048.4), separated by a T2A self-cleaving peptide, under the control of a CMV promoter, introduced into HMEC-1 cells via lentiviral transduction. This cell pool is designed to measure the uptake and recycling of human IgG antibodies (human FcRn does not bind mouse IgG). This cell pool has been validated with Nivolumab, Rituximab, Trastuzumab, anti-PCSK9 and an anti-IL-2Ra antibody. <p style="text-align: center;"><img src="{{media url="wysiwyg/82163.png"}}" alt="" width="650" height="400" /> Figure 1: Illustration of FcRn: IgG Recycling HMEC-1 Cell Pool mechanism of IgG FcRn-mediated recycling. <br />Cells are incubated with an antibody (IgG) of interest in a low pH Dilution Buffer, allowing FcRn to bind to IgG. Cells are then w

The HCMEC/D3 cell line represents an immortalized human cerebral microvascular endothelial cell line, extensively utilized in the study of the blood-brain barrier (BBB). This cell line was generated through the transduction of primary human cerebral microvascular endothelial cells with a lentiviral vector expressing human telomerase reverse transcriptase (hTERT), a crucial enzyme for maintaining telomere length and thereby promoting cellular longevity without transforming the cell phenotype. The introduction of hTERT helps these cells to bypass the replicative senescence that limits the lifespan of primary cells, allowing sustained propagation in culture. HCMEC/D3 cells retain key physiological and morphological characteristics of primary cerebral endothelial cells, making them a valuable model for in vitro studies of the BBB. These include the expression of tight junction proteins such as claudin-5, occludin, and zonula occludens-1, which are critical for maintaining barrier integrit

The HCMEC/D3 cell line represents an immortalized human cerebral microvascular endothelial cell line, extensively utilized in the study of the blood-brain barrier (BBB). This cell line was generated through the transduction of primary human cerebral microvascular endothelial cells with a lentiviral vector expressing human telomerase reverse transcriptase (hTERT), a crucial enzyme for maintaining telomere length and thereby promoting cellular longevity without transforming the cell phenotype. The introduction of hTERT helps these cells to bypass the replicative senescence that limits the lifespan of primary cells, allowing sustained propagation in culture. HCMEC/D3 cells retain key physiological and morphological characteristics of primary cerebral endothelial cells, making them a valuable model for in vitro studies of the BBB. These include the expression of tight junction proteins such as claudin-5, occludin, and zonula occludens-1, which are critical for maintaining barrier integrit

Please note this product may be subject to fees, we invite you to contact your local office. Expanded Human Peripheral Blood NK Cells are NK cells enriched and expanded from human PBMCs using the NK Expansion Kit (BPS Bioscience #78927), a K562 feeder cell-based system for about 2-3 weeks, and cryopreserved. Human Peripheral Blood NK Cells are >90 % pure NK cells (CD3^-CD56⁺ cells), as measured by flow cytometry analysis. They can be used in NK cytotoxicity assays or ADCC (antibody-dependent cellular cytotoxicity), after thaw and recovery in NK Cell Basal Medium for 24 hours or can be further expanded using the BPS Bioscience NK Expansion Kit (BPS Bioscience #78927).

Please note this product may be subject to fees, we invite you to contact your local office. Firefly Luciferase CD20 Knockout Raji Cell Line is a Raji cell line in which CD20 (B-lymphocyte antigen CD20, or MS4A1) has been genetically removed from Raji cells using CRISPR/Cas9 genome editing, while also constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. This cell line was generated by using Firefly Luciferase Lentivirus (BPS Bioscience #79692) on CD20 Knockout Raji Cell Line (BPS Bioscience #82622). This cell line has been validated by genome sequencing, flow cytometry, and luciferase activity measurement.

Please note this product may be subject to fees, we invite you to contact your local office. Firefly Luciferase CD19/CD20 Double Knockout Raji Cell Line is a Raji cell line in which CD19 (Cluster of Differentiation 19, B lymphocyte surface antigen B4, or CVID3) and CD20 (B-lymphocyte antigen CD20, or MS4A1) have been genetically removed from Raji cells using CRISPR/Cas9 genome editing, while also constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter. This cell line was generated by using Firefly Luciferase Lentivirus (BPS Bioscience #79692) on CD19/CD20 Double Knockout Raji Cell Line (BPS Bioscience #82623). This cell line has been validated by genome sequencing, flow cytometry and luciferase activity measurement.

Please note this product may be subject to fees, we invite you to contact your local office. The NK Cell Cytotoxicity Luciferase Assay Kit (NALM6) is designed to determine the cytotoxicity profile of NK (Natural Killer) cells towards the Firefly Luciferase NALM6 Cell Line. It uses the luminescence signal from Firefly Luciferase NALM6 Cell Line to measure the number of live target cells within a mixed cell population of NK and Firefly Luciferase NALM6 cells. The kit contains NK Cells, Firefly Luciferase NALM6 Cell Line, cell culture media and One-Step™ Luciferase Assay System.

Please note this product may be subject to fees, we invite you to contact your local office. The NK Cell Cytotoxicity Luciferase Assay Kit is designed to determine the cytotoxicity profile of NK (Natural Killer) cells towards a target cell line expressing Firefly luciferase. It uses the luminescence signal from Firefly luciferase expressing target cells to measure the number of live target cells within a mixed cell population of NK and target cells. The kit contains NK Cells, Thaw media, and One-Step™ Luciferase Assay System.