Labelling

Many small fluorescent probes are used to monitor lysosomes in living cells. Dojindo’s LysoPrime Deep Red overcomes known problems with fluorescent lysosome probes, such as lack of specificity for lysosomes and staining dependent on the lysosomal pH. In addition, the high-retentivity of LysoPrime Deep Red enables long-term imaging experiments.

Live imaging using small molecule fluorescent probes has been widely used for lysosomal live cell analysis, but the low specificity and retention ability due to pH change have been cited as issues. The pHLys Red is a small molecule fluorescent probe with high lysosomal specificity and sensitivity to pH changes, enabling a more accurate analysis of lysosomal pH in live cells. It is also applicable to experiments that require long-term imaging due to its high retention ability.

Live imaging using small molecule fluorescent probes has been widely used for lysosomal live cell analysis, but the low specificity and retention ability due to pH change have been cited as issues. The pHLys Red is a small molecule fluorescent probe with high lysosomal specificity and sensitivity to pH changes, enabling a more accurate analysis of lysosomal pH in live cells. It is also applicable to experiments that require long-term imaging due to its high retention ability.

This kit includes lysosome staining dyes, pHLys Red (pH dependent), and LysoPrime Green (pH-independent). The pHLys Red and LysoPrime Green accumulate in the intact lysosomes. The fluorescence intensity of pHLys Red is enhanced as the acidity increases, and weak fluorescence is observed when lysosomes are neutralized due to the lysosomal dysfunction. On the other hand, LysoPrime Green gives stable emissions even lysosomes are neutralized. Lysosomal pH and lysosomal mass can be measured by combining these two dyes.

High data variability, high fluorescent background, and self-oxidation by observation light are the major challenges in ROS detection. Especially the self-oxidation may cause a false response, however Dojindo Laboratories' ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- can solve these problems perfectly. The dye that is employed in this kit allows ROS detection with higher sensitivity than DCFH-DA; It does not leak from cells because the fluorescent dye can immobilize protein via a chemical bond, and it is resistant to photo-oxidation compared with DCFH-DA. Moreover, the Loading Buffer in the kit maintains cellular health during assays.

Cy3.5 NHS ester is a fluorophore featuring an NHS ester. The NHS ester is reactive towards amine groups to form a stable amide bond. Cy3.5 is a cyanine dye with excitation and emission maxima at 576 nm and 603 nm respectively.

Cy3.5 carboxylic acid is a fluorophore featuring a carboxylic acid. The carboxylic acid is readily attacked by amines to form a stable amide bond. Cy3.5 is a cyanine dye with excitation and emission maxima at 576 nm and 603 nm respectively.

Pyrene PEG4 Methyl tetrazine is a pyrene labeled PEG linker for the surface functionalization of carbon nanotube, graphene, graphene oxide, and reduced graphene oxide, which can be labled to other macromolecules via click chemistry with TCO. Strong pi-pi stacking is formed between the pyrene and the aromatic rings on single and multi-walled carbon nanotubes. Reagent grade, for research use only.