Nucleic Acids

Rat Brain Microvascular Endothelial Cell RNA (RBMVEC RNA), Adult

Rat Brain Microvascular Endothelial Cell RNA (RBMVEC RNA), Adult

Propargyl-PEG3-CE-phosphoramidite is a linker featuring a terminal alkyne ligated to a phosphoramidite group. This phosphoramidite may be applied in solid-phase oligonucleotide synthesis to add an alkyne to the oligonucleotide sequence or other related phosphoramidite chemistry. The alkyne may be used in copper click chemistry with azides to form stable triazoles with the target molecule.

The TAMRA phosphoramidite (6-isomer) is employed in the synthesis of oligonucleotides labeled at the 5’ end with TAMRA. TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis due to the availability of equipment with a TAMRA detection channel.

TE-L-Tyrosine (TET) serves as a precursor for O-(2-[18F]fluoroethyl)-L-tyrosine (FET), an F18-labeled amino acid tracer extensively employed in positron emission tomography and computed tomography (PET/CT). FET facilitates amino acid PET imaging in clinical settings, aiding in tasks like assessing amino acid transport in brain tumors and distinguishing tumor tissues.

5-Vinyl-2’-deoxyuridine(VdU) is a synthetic thymidine analog used to study DNA synthesis and cell proliferation. It incorporates into DNA during the S-phase, replacing natural thymidine. VdU-labeled DNA can be detected with biotin or fluorescent dyes using a copper-free alkene-tetrazine reaction (IEDDA). This makes it a potential alternative to BrdU and EdU for DNA purification and cell imaging.

5-Vinyl-uridine (VU) is a uridine derivative with a terminal alkene group used to monitor RNA transcription. VU is incorporated into new RNA by RNA polymerases, replacing endogenous uridine. VU-labeled RNA can be rapidly detected with high sensitivity via IEDDA cycloaddition between the vinyl group and labeled tetrazines. This allows detection through methods such as fluorescent microscopy or flow cytometry, providing insights into cellular transcription levels.