DNA & Clones

The pE-SUMOpro vector is for the expression of recombinant SUMO fusion proteins (containing an N-terminal His6 tag) in E. coli. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with kanamycin resistance facilitating plasmid selection and stability.

The pE-SUMOpro3 vector is for the expression of recombinant SUMO3 fusion proteins (containing an N-terminal His6 tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with kanamycin resistance facilitating plasmid selection and stability.

The pE-SUMOpro3 vector is for the expression of recombinant SUMO3 fusion proteins (containing an N-terminal His6 tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with ampicillin resistance facilitating plasmid selection and stability.

The pE-SUMOstar vector is for the expression of recombinant SUMOstar fusion proteins (containing an N-terminal His6 tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with kanamycin resistance facilitating plasmid selection and stability.

The pE-SUMOstar vector is for the expression of recombinant SUMOstar fusion proteins (containing an N-terminal His₆ tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with ampicillin resistance facilitating plasmid selection and stability.

pI-SUMOstar is used for intracellular expression of SUMOstar fusion proteins (containing an N-terminal His6 tag) in baculovirus expression systems (BEVS). Bacmid generation from this plasmid requires DH10bac for transposition into the baculoviral genome. Expression of your protein of interest (POI) is driven by the late stage viral polyhedrin promoter (<em>polh</em>). pI-SUMOstar contains an ampicillin marker for propagation in <em>E. coli</em>.

pI-secSUMOstar is used for expression and secretion of SUMOstar fusion proteins (containing an N-terminal His6 tag) in baculovirus expression vector systems (BEVS). Bacmid generation from this plasmid requires the use of DH10bac for transposition into the baculoviral genome. Expression of fusion proteins is driven by the late stage viral polyhedrin promoter (<em>polh</em>). Your protein of interest (POI) is targeted for processing and secretion through fusion with the secretion signal sequence from the envelope surface glycoprotein 67 (gp67) of <em>Autographa californica</em>.  pI-secSUMOstar contains an ampicillin marker for propagation in <em>E. coli</em>.

The pM-SUMOstar vector allows for cytosolic expression of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  SUMO fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).

The pM-secSUMOstar vector allows for expression and secretion of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  Proteins are directed to the secretory pathway by the mouse IgG kappa secretory signal, which precedes a His6 tag. SUMOstar fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).