DNA & Clones

Fragmented DNA derived from Escherichia coli (E. coli) can be used as a spike-in control for experimental normalization in Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUT&RUN normalization was originally performed with carryover E. coli DNA from MNase preparation [1], however pure preps of enzyme require post hoc DNA spike-in. CUTANA™ E. coli Spike-in DNA contains sufficient material for 100-200 CUT&RUN reactions (range for high and low abundance targets).

Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated is a double-stranded DNA fragment with 26 bp linkers on either end of a 147 bp 601 Widom sequence, identified by Lowary and Widom using the SELEX method [1]. The 601 sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. The DNA contains a 5’ biotin-TEG group.

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. This negative control does not contain DpnII restriction sites. When paired with the positive control DNA (Catalog No. 18-4101) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes/

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment. The sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 DNA). The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control (EpiCypher 18-4100), these controls illustrate the migration range for the Restriction Enzyme Assay. For more information, see the EpiDyne Nucleosome Remodeling Assay Tech Note: epicypher.com/resources./technical-notes/

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5’ biotin-TEG group. This negative control does not contain DpnII restriction sites. When paired with the positive control DNA (EpiCypher 18-4111) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes/

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5’ biotin-TEG group. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control DNA (EpiCypher 18-4110) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes/

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group and two GATC restriction enzyme sites. This sequence includes the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5’ biotin-TEG group. When assembled onto a nucleosome, the GATC sites are shielded within the 601 sequence but can be made accessible for restriction enzyme cleavage after nucleosome remodeling. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes/

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, 50-N-66, Biotinylated is a 263 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see EpiCypher 18-0005), as well as both a 5’ and 3’ acceptor sequence to accommodate the histone octamer subsequent remodeling. ST601-GATC1,2,3, 50-N-66 DNA has restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1, 50-N-66, Biotinylated is a 263 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see EpiCypher 18-0005), as well as both a 5’ and 3’ acceptor sequence to accommodate the histone octamer subsequent remodeling. ST601-GATC1, 50-N-66 DNA has restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.