Viral Vectors & Particles

Nuclear mCherry Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain a nuclear mCherry, with nuclear localization sequences (NLS) at both the N- and C-terminus of mCherry, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. mCherry expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

The Firefly Luciferase-Nuclear eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain firefly luciferase and nuclear eGFP (Luc2-P2A-nuclear eGFP) driven by an EF1A promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). The nuclear eGFP has NLS sequences at the C-terminus. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.

The NFĸB Luciferase-eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and nondividing cells. The particles contain a firefly luciferase and eGFP (enhanced green fluorescent protein) cassette driven by the NFĸB (nuclear factor kappa-light-chain-enhancer of activated B cells) response element located upstream of the minimal TATA promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). After transduction, activation of the NFĸB signaling pathway in the target cells can be monitored by measuring luciferase activity or/and eGFP expression.

The CDH17 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with human CDH17 (cadherin 17) (NM_004063.4) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection marker.

The PTK7 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with human PTK7 (Protein Tyrosine Kinase 7) (NM_002821.5) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection marker.