Chemicals & Small Molecules

SPY650-FastAct_X (Also known as SiR-XActin) is our most advanced live cell actin imaging probe. It labels highly dynamic actin filaments as well as the more static ones. SPY650-FastAct_X is a bright, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin. SPY650-FastAct_X can label highly dynamic cellular actin structures but lacks undesired F-actin stabilization and toxic side effects. It does not require any transfection and works just by adding the probe to the cells and perform imaging a couple of hours later. Product contains 1 vial of SPY650-FastAct_X in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings)

SPY555-FastAct_X is a bright, far red, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. SPY555-FastAct_X’s carefully optimized actin ligand allows to label Factin in live cells with very high specificity. SPY555-FastAct_X can label very fast actin dynamics but lacks undesired F-actin stabilization side effect. The probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY555-FastAct_X is based on a low phototoxicity, bright & highly photostable SPY555 fluorophore which is far superior than fluorescent proteins. SPY555-FastAct_X enables multicolor imaging with SPY505, SPY595, PY620, SPY650, SPY700, GFP or mcherry. SPY555-FastAct_X can be imaged with standard Cy3filterset. It can be used for widefield, confocal, SIM or STED imaging of living cells and tissue. Contains 1 vial of SPY555-FastAct_X (lyophilized)