Labelling

Fmoc-Lys(CR110)-OH is a green fluroescent molecule with two Boc protecting groups and terminal carboxylic acid and Fmoc moieties. The protected amines can be deprotected under mild acidic conditions. It has an excitation at 502 nm and emission at 527 nm. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. The Fmoc group can be deprotected under basic conditions to obtain the free amine which can be used for further conjugations.

Sulfo-Cy3-amide-C6-maleimide is a water soluble, thiol-reactive dye for labeling with a hydrophilic sulfo-Cyanine3 fluorophore. The maleimide group will react with a thiol group to form a covalent bond.

BP Fluor 647 Alkyne is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The dye is water soluble and pH-insensitive from pH 4 to pH 10. The dye has 4 sulfonate groups which make it highly water soluble and cause less aggregation in the aqueous solution. BP Fluor 647 is used for protein and antibody labeling, or nucleic acid applications with high labeling density.

mutaFISH&trade; EGFR T790M T790wt RNA Probes is designed to detect human EGFR T790M gene mutation on single strand RNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.

mutaFISH&trade; EGFR L858R L858wt Ex19wt RNA Probes is designed to detect human EGFR L858R gene mutation and EGFR exon 19 deletion on single strand RNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.

mutaFISH&trade; MYCNwt DNA Probes is designed to identify human MYCN gene amplification on dsDNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.

mutaFISH&trade; AR-V7 ARwt RNA Probes is designed to detect AR wildtype and AR-V7 on single strand RNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.

mutaFISH&trade; CEP8wt DNA Probes is designed to identify human CEP8 amplification on dsDNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.

mutaFISH&trade; CEP7wt DNA Probes is designed to identify human CEP7 amplification on dsDNA in cells using padlock probe and <I>in situ</i> rolling-circle amplification technology.