Labelling

BDP FL-PEG7-Methyltetrazine is a BDP dye linker containing a methyltetrazine group and a hydrophilic PEG spacer arm. Methyltetrazine is reactive toward trans-cyclooctenes. The additional methyl on the methyltetrazine enhances the stability of the group. The hydrophilic PEG spacer arm increases water solubility, membrane permability, and conjugation efficiency. This dye is used for protein and peptide labeing and is photostable. BDP FL-PEG7-Methyltetrazine is a green-fluorescent dye and has several characteristics that make it potentially superior in some applications, including high extinction coefficient, high fluorescence quantum yield, narrow emission bandwidth, red shift in fluorescence emission at high dye concentrations, relatively long excited-state lifetime, etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

AMCA-PEG8-DBCO is a AMCA dye containing a reactive DBCO group and a hydrophilic PEG spacer arm with excitation/emission maximum of 345/450 nm. DBCO reacts with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalysts. It could be used as a quantum yield standard. Hydrophilic PEG spacer increases solubility in aqueous media and reduces steric hindrance during binding. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Fluorescein-PEG2-Biotin is a xanthene dye with excitation/emission maximum of 494/517 nm and a terminal biotin group with hydrophilic PEG spacer arm. The biotin group is effective at binding proteins such as binding to avidin, streptavidin or neutravidin. The hydrophilic PEG spacer can increase aqueous solubility of the biotin conjugated molecules. It also can also help minimize steric hindrance and provide extra flexibility with the binding to stravidin and avidin molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

BDP FL-PEG8-DBCO is a BDP FL linker containing a reactive DBCO group and a hydrophilic PEG spacer arm. DBCO reacts with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalysts. BDP FL-PEG8-DBCO is a bright and photostable thiol-reactive dye for protein labeling, peptide modification, and can replace fluorescein for microscopy. The hydrophilic PEG spacer arm increases water solubility, membrane permeability, and conjugation efficiency. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

FAM Phosphoramidite, 6-isomer (hydroxyprolinol) serves as a versatile reagent for 5’ and internal labeling of oligonucleotides. It facilitates the synthesis of fluorescein-labeled strands. The 6-aminohexanoate linkage acts as a spacer between the nucleotide skeleton and the functional group. Additionally, this reagent features a dimethoxythrityl protection group, enabling purification via reversed-phase HPLC or cartridge methods.

TCO Phosphoramidite C6 acts as a versatile reagent for TCO-modified oligonucleotides, introducing a TCO moiety into substrates with primary or secondary hydroxyl groups. Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). The resulting TCO-Tetrazine ligation offers ultrafast kinetics, selectivity, and long-term stability in aqueous media, making it valuable for low-concentration applications like protein-protein conjugations.

DAPI (hydrochloride) is a blue-emitting fluorescent dye that strongly binds to adenine-thymine-rich regions in DNA. Widely used in fluorescence microscopy and flow cytometry, DAPI serves as a nuclear counterstain. When bound to double-stranded DNA, DAPI exhibits ~20-fold fluorescence enhancement, with an absorption peak at 358 nm and emission peak at 461 nm. It also binds to RNA, albeit with less intensity, causing its emission spectrum to shift to ~500 nm.

Hoechst 33258 is a blue-emitting fluorescent dye that selectively binds to adenine-thymine-rich regions in double-stranded DNA. Its fluorescence is enhanced by AT-rich DNA strands. With excitation/emission maxima at 351/463 nm, it exhibits a considerable Stokes shift. Although it can enter living cells, its permeability is lower than Hoechst 33342. Notably, Hoechst 33258 is less toxic than DAPI, ensuring better cell viability.

Hoechst 33342 is a blue-emitting fluorescent dye which selectively binds to adenine-thymine-rich regions in double-stranded DNA. With excitation/emission maxima at 351/461 nm, it exhibits a considerable Stokes shift. Notably, Hoechst 33342 is less toxic than DAPI, ensuring better cell viability. It is widely used in fluorescence microscopy and flow cytometry for staining chromosomes and nuclei in live and fixed cells, particularly to distinguish condensed pycnotic nuclei in apoptotic cells and for cell sorting.